IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Very odd abstract found. Any comments?

ufotruth at ix.netcom.com ufotruth at ix.netcom.com
Wed Sep 9 19:34:47 EST 1998


Everyone,

I found the following abstract on the web. I was wondering if anyone
had any comments about it. From everything I have read before it
seemed like it had been proven that cells taken from younger
individuals did indeed divide more times than cells from older
individuals.

Could the experiment mentioned in the following abstract be flawed or
in error? Hey, it might be for real but it seems to me that it has
already been tested many times that cells from younger people divide
more times than from older people....

Anyway.. Here is the abstract.

Best Regards,
William

----

Vol. 95, Issue 18, 10614-10619, September 1, 1998


Cell Biology
Relationship between donor age and the replicative lifespan of human
cells in culture: A reevaluation 

(cell proliferation/cell senescence/aging) 

Vincent J. Cristofalo*,<Picture: dagger >, Robert G. Allen*, Robert J.
Pignolo*, Bernard G. Martin*, and Jeanne C. Beck<Picture: Dagger > 

* Center for Gerontological Research, Allegheny University of the
Health Sciences, 2900 Queen Lane, Philadelphia, PA 19129; and
<Picture: Dagger > Coriell Institute for Medical Research, 401 Haddon
Avenue, Camden, NJ 08103 

Communicated by Harry Rubin, University of California, Berkeley, CA,
July 6, 1998 (received for review May 1, 1998) 

Normal human diploid fibroblasts have a finite replicative lifespan in
vitro, which has been postulated to be a cellular manifestation of
aging in vivo. Several studies have shown an inverse relationship
between donor age and fibroblast culture replicative lifespan;
however, in all cases, the correlation was weak, and, with few
exceptions, the health status of the donors was unknown. We have
determined the replicative lifespans of 124 skin fibroblast cell lines
established from donors of different ages as part of the Baltimore
Longitudinal Study of Aging. All of the donors were medically examined
and were declared "healthy," according to Baltimore Longitudinal Study
of Aging protocols, at the time the biopsies were taken. Both long-
and short-lived cell lines were observed in all age groups, but no
significant correlation between the proliferative potential of the
cell lines and donor age was found. A comparison of multiple cell
lines established from the same donors at different ages also failed
to reveal any significant trends between proliferative potential and
donor age. The rate of [3H]thymidine incorporation and the initial
rates of growth during the first few subcultivations were examined in
a subset of cell lines and were found to be significantly greater in
fetal lines than in postnatal lines. Cell lines established from
adults did not vary significantly either in initial growth rate or in
[3H]thymidine incorporation. These results clearly indicate that, if
health status and biopsy conditions are controlled, the replicative
lifespan of fibroblasts in culture does not correlate with donor age. 


------------------------------------------------------------------------
<Picture: dagger >   To whom reprint requests should be addressed at:
Center for Gerontological Research, Allegheny University of the Health
Sciences, Philadelphia, PA 19129. 




Copyright © 1998 by The National Academy of Sciences
 0027-8424/98/9510614-6$2.00/0 






More information about the Ageing mailing list

Send comments to us at biosci-help [At] net.bio.net