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Very odd abstract found. Any comments?

James james at nospam.com
Wed Sep 9 22:55:47 EST 1998

Thanks for the abstract - I don't search the literature as much as I
should.  Looks like he explains the apparent paradox pretty well.  It is
true that the variance in these types of studies is very high and the data
could have been skewed by unknown factors.  I would think that this data
should be better since he examines so many cell lines, and since he has
access to the data from this longitudinal study (which is one of only two
that I know about - no one ever had this type of data before).

Sounds to me like he repeated old experiments with what should be much
better data, and got different results.  That could surely happen, so I am
leaning towards believing it...  But I don't see how cells that are not
expressing telomerase are maintaining the replicative potential in vivo
(which is the conclusion I draw from the fact that their replicative
capacities do not differ significantly when grown in culture), and then
senescing uniformly in vitro.

Were I to follow this line of reasoning to it's logical conclusion the
answer I arrive at is that the senescence we see in fibroblasts in vitro is
an artifact of the culture conditions and that they really do not senesce in
vivo. But that's a little too off the beaten path for me to accept at the
moment.  Maybe it's just late...  Anyone else have any thoughts?

ufotruth at ix.netcom.com wrote:

> Everyone,
> I found the following abstract on the web. I was wondering if anyone
> had any comments about it. From everything I have read before it
> seemed like it had been proven that cells taken from younger
> individuals did indeed divide more times than cells from older
> individuals.
> Could the experiment mentioned in the following abstract be flawed or
> in error? Hey, it might be for real but it seems to me that it has
> already been tested many times that cells from younger people divide
> more times than from older people....
> Anyway.. Here is the abstract.
> Best Regards,
> William
> ----
> Vol. 95, Issue 18, 10614-10619, September 1, 1998
> Cell Biology
> Relationship between donor age and the replicative lifespan of human
> cells in culture: A reevaluation
> (cell proliferation/cell senescence/aging)
> Vincent J. Cristofalo*,<Picture: dagger >, Robert G. Allen*, Robert J.
> Pignolo*, Bernard G. Martin*, and Jeanne C. Beck<Picture: Dagger >
> * Center for Gerontological Research, Allegheny University of the
> Health Sciences, 2900 Queen Lane, Philadelphia, PA 19129; and
> <Picture: Dagger > Coriell Institute for Medical Research, 401 Haddon
> Avenue, Camden, NJ 08103
> Communicated by Harry Rubin, University of California, Berkeley, CA,
> July 6, 1998 (received for review May 1, 1998)
> Normal human diploid fibroblasts have a finite replicative lifespan in
> vitro, which has been postulated to be a cellular manifestation of
> aging in vivo. Several studies have shown an inverse relationship
> between donor age and fibroblast culture replicative lifespan;
> however, in all cases, the correlation was weak, and, with few
> exceptions, the health status of the donors was unknown. We have
> determined the replicative lifespans of 124 skin fibroblast cell lines
> established from donors of different ages as part of the Baltimore
> Longitudinal Study of Aging. All of the donors were medically examined
> and were declared "healthy," according to Baltimore Longitudinal Study
> of Aging protocols, at the time the biopsies were taken. Both long-
> and short-lived cell lines were observed in all age groups, but no
> significant correlation between the proliferative potential of the
> cell lines and donor age was found. A comparison of multiple cell
> lines established from the same donors at different ages also failed
> to reveal any significant trends between proliferative potential and
> donor age. The rate of [3H]thymidine incorporation and the initial
> rates of growth during the first few subcultivations were examined in
> a subset of cell lines and were found to be significantly greater in
> fetal lines than in postnatal lines. Cell lines established from
> adults did not vary significantly either in initial growth rate or in
> [3H]thymidine incorporation. These results clearly indicate that, if
> health status and biopsy conditions are controlled, the replicative
> lifespan of fibroblasts in culture does not correlate with donor age.
> ------------------------------------------------------------------------
> <Picture: dagger >   To whom reprint requests should be addressed at:
> Center for Gerontological Research, Allegheny University of the Health
> Sciences, Philadelphia, PA 19129.
> Copyright © 1998 by The National Academy of Sciences
>  0027-8424/98/9510614-6$2.00/0

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