27'th update on fly longevity experiments

Doug Skrecky oberon at vcn.bc.ca
Mon Feb 7 00:06:08 EST 2000


    This is the 27'th update on my fly longevity experiments. Run #22
investigated the effect on aging of the artichoke, and leucoselect
grape extract supplements, which had reduced ethanol induced mortality
in run  #21.  Also some supplements (eg sage) were retested, which had
increased longevity in earlier experiments, that had not included citric
acid to eliminate pathogen growth. I included blueberry juice as well,
since this appears to have a potent brain antiaging effect in rodents.
    No benefits on 50% survivals were found, a few supplements
headed by artichoke extended maximum lifespan. This increased
maximum survival to a surprising degree, from 62 days to 95 days. I
can think of two possible explanations for this strange finding. Either
artichoke genuinely slows fly aging, or it slows fly food deterioration.
This second possibility exists due to the fact I do not change the fly
food after a run is started. Although, protein, carbohydrates, and
minerals may not deteriorate to a serious degree, in the absence
of pathogen growth, vitamins are known to be unstable in solution.
Thus it is probable that aged flies were suffering from vitamin
deficiencies. If artichoke extract somehow helped prevent the
deterioration of these vitamins, then this might account for the positive
impact on maximum survival, without any advantage on 50% survival.
    The results with sage were quite disappointing, and indicate that
most of the longevity promoting effects of sage found in earlier
experiments were due to an antipathogen effect. Thus these old experiments
are of doubtful value.
    Additionally this run discovered a new phenomena, which I had never
seen in any of my previous experiments. On the day 17 census the flies
in  the prune juice bottle, showed almost complete absence of any movement.
They were joined on the day 24 census by flies in the blueberry bottle. I
usually had to examine the flies in these bottles under magnification to
be determine whether they were dead or alive. I speculate their extremely
low metabolic rate might be of some interest to cancer patients. Lowered
body temperature is known to inhibit tumor growth. If high doses of these
juices could lower the metabolic rate in humans, they might reduce body
temperature, and thereby extend the lives of cancer patients.

Run #22                    Percent Survival on Day
supplement          17 24 30 40 50 56 62 68 73 81 86 90 95
control             76 59 59 52 14 14  7  0  -  -  -  -  -
aloe vera 450 mg    47 40 40 30 13  7  3  0  -  -  -  -  -
artichoke 250 mg    77 73 65 58 35 38 31 27 23 15  8  4  4
artichoke 500 mg    64 52 36 30 18 18 18  6  0  -  -  -  -
blueberry juice     80 70 43 47 20 23 10 10  0  -  -  -  -
leucoselect 100 mg  67 58 50 50 46 29 13  8  8  8  4  0  -
leucoselect 200 mg  65 41 41 35 29 29 29 24 12  0  -  -  -
coriander 1/16 tsp  76 52 45 41 17 14  3  3  3  3  3  3  0
hawthorn 500 mg     81 69 50 31 15  8  4  0  -  -  -  -  -
prune juice         50 42 33 17 17  8  8  0  -  -  -  -  -
royal jelly 500 mg  67 40 33 20 13 13  7  0  -  -  -  -  -
sage 1/4 tsp        29 29 29 29 14 14 14  7  7  0  -  -  -
E succinate 100 IU  94 72 61 39 22 11 11 11  0  -  -  -  -

    The high early survival with vitamin E succinate was mildly interesting.
So in run #23, I tested this on alcohol induced mortality. This proved to
be a dud, and I am inclined to think the good initial result in run #22 was
mere chance.
    Note that once again leucoselect comes through with good results. A higher
dosage of this proved to be optimal in retarding alcohol induced mortality, 
than that found to be best for anti-aging in run #22. The higher free 
radical generation in alcohol stressed flies may account for this.

Run #23                       Percent Survival on Day
supplement            3   5   8  12  15  21  25
wine control         93  80  60  53   7   0   -
+leucoselect 100 mg  80  70  60  50  30  10   0
+leucoselect 200 mg 100 100  83  58  25   8   8
+E acetate 100 IU    83  83  67  33   0   -   -
+E succinate 100 IU  80  70  20   0   -   -   -
+E succinate 200 IU 100  58   0   -   -   -   -
+E succinate 400 IU  79  64  21   0   -   -   -






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