Nature Article: Apoptosis

Dr. Sydney Shall sydney.shall at kcl.ac.uk
Tue Jan 4 12:15:24 EST 2000



Aubrey de Grey wrote:

> Paul Brookes wrote:
>
> > The assumption by everyone has been that what they're measuring is
> > apoptosis.  What they in fact measure is cell viability by Trypan Blue
> > exclusion.  No TUNEL staining, DNA laddering, or caspase activation.
> > The WT cells shown in Fig 2C don't look particularly apoptotic.   Thus
> > it could be apoptosis, necrosis, or anything on the spectrum in between.
>
> Very good point -- but in fact they do mention TUNEL staining (page 310,
> left column, line 3).
>
> > The notion that "apoptosis is something to be prevented in order to
> > prolong lifespan" is certainly not proven by this paper.
>
> I absolutely agree.
>
> > What they have neatly shown is that prevention of cell death and
> > prolonged lifespan are linked, which isn't exactly a great leap of
> > faith for anyone.
>
> Hm, well it surprised me!
>
> > By the way .... do MEF's have many mitochondria?

It depends on how many are many?  They do have a lot, and are very active in
oxygen consumption.  Less than muscle, but still quite a lot.

Probably not helpful.  But there is considerable evidence for oxidative
damage if that helps any.

Cheers,

Sydney Shall

>
>
> I don't know.
>
> Aubrey de Grey

-- Dr. Sydney Shall, Department of Molecular Medicine, King's College School
of Medicine & Dentistry, The Rayne Institute, 123 Coldharbour Lane, LONDON,
SE5 9NU, TEL: 01 71 346 3126; FAX: 01 71 733 3877, E-Mail:
sydney.shall at kcl.ac.uk

---





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