29'th update on fly longevity experiments

Doug Skrecky oberon at vcn.bc.ca
Mon Jun 12 11:41:56 EST 2000


    This is the 29'th update on my fly longevity experiments.
In run #26 I checked to see if an alkaline potassium salt can
help reduce alcohol toxicity - result was negative. I also
checked to see whether citric acid exerts any toxic side effects.
I need to add 1/4 tsp to help prevent pathogen growth, but
was curious to see if citric acid might itself reduce longevity,
when enough alcohol is added to already eliminate pathogen
growth. Citric acid proved to be non-toxic.

Run #26                Percent Survival on Day
supplement        6   9  13  19  24  31  38  47  54  63
_______________________________________________________
25% vodka        86  79  61  46  29  25  14   7   7   0
+K bicarbonate   88  81  75  38  13   6   0   -   -   -
+citric 1/8 tsp  78  63  56  41  30  11  11   7   4   0
+citric 1/4 tsp  83  65  61  52  26  22  13   9   9   4

    In prior runs I have found pycnogenol exerts a temporary
survival promoting effect. I was wondering if this effect was 
short term  because of the antioxidants deteriorating.
In run #27 I check to see if prior exposure for 2 weeks to 200 mg
pycnogenol eliminates the benefit when fresh pycnogenol is
then added. Unfortunely there were not enough flies to include 
the bottle using the old pycnogenol laced fly food. In any case 
due to the low mortality at the day 8 census, the results are 
not robust enough to be decisive.

Run #27                 Percent Survival on Day
supplement              8  11  15  24  32  38  47
_________________________________________________
control/control        80  80  76  64  40  16   4
control/pycnogenol     94  78  67  44  17  11   0
pycnogenol/control     79  75  50  17   8   4   0
pycnogenol/pycnogenol  84  63  53  26  16   0   -

    Finally in an interesting recent experiment I tested the
effect of one day exposure to 200 mg pycnogenol or wine on
the ability of flies to survive various lengths of time in my freezer.
After each period in the freezer, the flies had to be warmed up
at room temperature to see they could start moving again.
The poor result with alcohol was a surprise, and as a result no
further experiments with alcohol are planned. Also unexpected
was the apparent ability of pycnogenol to significantly boost 
hypothermia/freezing tolerance. 
    In additional to further longevity experiments I plan
additional "low-tech" cryobiology experiments. If I can find a way
for flies to survive long periods in the freezer this would be a
major advance, which might have commercial application
with regard to transplant organs for example. (I can dream.)

Freezer Run #1 Cumulative Percent Survival After
supplement     0  33  60  65 (minute episodes in freezer)
____________________________
control      100 100  13   0
pynogenol    100 100  48  24
wine          70  55   5   0







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