cytochrome c oxidase and senescence

Doug Skrecky oberon at vcn.bc.ca
Fri Mar 10 21:12:55 EST 2000


Citations: 1-2
<1>
Authors
  Begel O.  Boulay J.  Albert B.  Dufour E.  Sainsard-Chanet A.
Institution
  Centre de Genetique Moleculaire-Centre National de la Recherche Scientifique,
  91198 Gif-sur-Yvette Cedex, France.
Title
  Mitochondrial group II introns, cytochrome c
  oxidase, and senescence in Podospora anserina.
Source
  Molecular & Cellular Biology.  19(6):4093-100, 1999 Jun.
Abstract
  Podospora anserina is a filamentous fungus with a limited life span. It
  expresses a degenerative syndrome called senescence, which is always
  associated with the accumulation of circular molecules (senDNAs) containing
  specific regions of the mitochondrial chromosome. A mobile group II intron
  (alpha) has been thought to play a prominent role in this syndrome. Intron
  alpha is the first intron of the cytochrome c
  oxidase subunit I gene (COX1). Mitochondrial mutants that
  escape the senescence process are missing this intron, as well as the first
  exon of the COX1 gene. We describe here the first mutant of P. anserina that
  has the alpha sequence precisely deleted and whose
  cytochrome c oxidase activity is identical
  to that of wild-type cells. The integration site of the intron is slightly
  modified, and this change prevents efficient homing of intron alpha. We show
  here that this mutant displays a senescence syndrome similar to that of the
  wild type and that its life span is increased about twofold. The introduction
  of a related group II intron into the mitochondrial genome of the mutant does
  not restore the wild-type life span. These data clearly demonstrate that
  intron alpha is not the specific senescence factor but rather an accelerator
  or amplifier of the senescence process. They emphasize the role that intron
  alpha plays in the instability of the mitochondrial chromosome and the link
  between this instability and longevity. Our results strongly
  support the idea that in Podospora, "immortality" can be acquired not by the
  absence of intron alpha but rather by the lack of active
  cytochrome c oxidase.

<2>
Authors
  Sohal RS.  Sohal BH.  Orr WC.
Institution
  Department of Biological Sciences, Southern Methodist University, Dallas, TX
  75275, USA.
Title
  Mitochondrial superoxide and hydrogen peroxide generation, protein oxidative
  damage, and longevity in different species of flies.
Source
  Free Radical Biology & Medicine.  19(4):499-504, 1995 Oct.
Abstract
  The objective of this study was to further elucidate the role of oxidative
  stress in the aging process by determining whether or not the rates of
  mitochondrial superoxide anion radical and hydrogen peroxide (H2O2)
  production, the activity of cytochrome c
  oxidase, and the concentration of protein carbonyls are
  correlated with the life span potential of different species. A comparison
  was made among five different species of dipteran flies, namely, Drosophila
  melanogaster (fruit fly), Musca domestica (house fly), Sarcophaga bullata
  (flesh fly), Calliphora vicina (blow fly) and Phaenecia sericata (a species
  of blow flies), which range more than 2-fold in their life span potentials.
  The average life span potential of these species was found to be inversely
  correlated with the rates of mitochondrial superoxide and H2O2 production and
  with the level of protein carbonyls, and to be directly related to the
  activity of cytochrome c oxidase. The
  significance of these findings in context of the validity of the oxidative
  stress hypothesis of aging is discussed. It is inferred that longer life span
  potential in these insect species is associated with relatively low levels of
  oxidant generation and oxidative molecular damage. These results accord with
  our previous findings on different mammalian species.






More information about the Ageing mailing list