<html><div>The assumption by everyone has been that what they're
measuring is apoptosis. What they in fact measure is cell viability
by Trypan Blue exclusion. No TUNEL staining, DNA laddering, or
caspase activation. The WT cells shown in Fig 2C don't look
particularly apoptotic. Thus it could be apoptosis, necrosis,
or anything on the spectrum in between. </div>
<br>
<div>The notion that "apoptosis is something to be prevented in
order to prolong lifespan" is certainly not proven by this
paper. What they have neatly shown is that prevention of cell death
and prolonged lifespan are linked, which isn't exactly a great leap of
faith for anyone.</div>
<br>
<div>By the way .... do MEF's have many mitochondria?</div>
<br>
<div>PSB</div>
<br>
<br>
<font color="#000080">_________________________________________<br>
</font><font color="#FF0000"><b>Dr. Paul S.
Brookes.</b>
(brookes@uab.edu)<br>
</font><font color="#000080">UAB Department of Pathology,
G004 Volker Hall<br>
1670 University Blvd., Birmingham AL 35294 USA<br>
Tel (001) 205 934 1915 Fax (001) 205 934
1775<br>
<a href="http://peir.path.uab.edu/brookes" eudora="autourl">http://peir.path.uab.edu/brookes</a><br>
<br>
<b>The quality of e-mails can go down as well as up<br>
</font></b></html>
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