IUBio

DNA contamination of RNA preps

Enter your name here Enter.your.email.address.here at panda1.uottawa.ca
Wed Feb 7 15:24:03 EST 1996


Hi

I'm trying to get rid of residual DNA in my plant mitochondrial
 RNA preps.  I have tried a combination of precipitation in 2 M 
LiCl up to 3 times, followed by a DNAse treatment.  DNA fragments
 of up to 800 bp in length can be amplified using PCR following 
this treatment (fragments are known to be of DNA origin as they 
are not edited.  

I would greatly appreciate any suggestions!

Cathy Carrillo

Dept. of Biology
University of Ottawa




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