Dear Michel
I developed a method of grading the shells to quantify infestations
which gave quite good results for my MSc. thesis. See:
Handley, S.J. & Bergquist, P.R. 1997a. Spionid polychaete
infestations of intertidal Pacific oysters Crassostrea gigas
(Thunberg), Mahurangi Harbour, Northern New Zealand.
Aquaculture. 153: 191-205.
As far as extracting spionids, below is a previous message in reply
to a similar request a few years ago. It would be worthwhile
checking Geoff's Annelida archives on the web page for other
replies around that time.
Date sent: Thu, 17 Jul 1997 11:34:27 GMT+1200
Subject: Re: Extracting polydorids
> I would be grateful for any suggestions on how to extract
polydorids
A method I have used to extract spionids from oysters is the use of
the vermifuges: 0.5% phenol and/or 0.25% di-dichlorobenzene in
seawater. The worms are expelled from their host if left overnight in
these solutions. Caution is advised working with di-
chlorobenzene...
For further reading on vermifuges and acid dissolution of coral see:
MacKenzie, C.L.; Shearer, L.W. 1959: Chemical control of Polydora
websteri and other annelids inhabiting oyster shells. Proceedings -
National Shellfisheries Association 50: 105-111.
Brock, R.E. and Brock, J.H. 1977: A method for quantitatively
assessing the infaunal community in coral rock. Limnol.Oceanogr.
22:948-951, .
Regards....Sean
_________________________________________
Dr Sean Handley Marine Ecologist
National Institute of Water & Atmospheric Research
(NIWA) P.O. Box 893, Nelson, New Zealand
Ph (64 03)5481715 DDI (64 03) 545 7735
Fax (64 03)5481716 http://www.niwa.cri.nz/
_________________________________________
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