Preservative for environmental SEM

Sean Handley s.handley at
Tue Sep 12 17:04:20 EST 2000

Another method I tried successfully was that of freeze dehydration... see: 
Veltkamp, C. J., J. C. Chubb, et al. (1994). “A simple freeze dehydration  
method for studying epiphytic and epizoic communities using the scanning  
electron microscope.” Hydrobiologia 288: 33-38.  

No nasty chemicals, but careful and quick washing with distilled water is  
needed to avoid osmotic blowouts. 

Regards... Sean 

Dr Sean Handley      Marine Ecologist National Institute of Water & 
Atmospheric Research (NIWA) P.O. Box 893, Nelson, New Zealand Ph (64 
03)5481715    DDI (64 03) 545 7735 Fax (64 03)5481716  <s.handley at>

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