The Centre for Marine Biodiversity and Biotechnology at Heriot-Watt
University, Edinburgh will be running its course in Molecular Systematics for
Taxonomists again this March. Attached is a pamphlet detailing the
programme, costs and a booking form. Please send completed forms and
any further correspondance connected with the course directly to Dr. Paul
Kingston, at P.F.Kingston at hw.ac.uk , the email address given in the flier.
Also, please pass this information on to any of your colleagues who may also
be interested in attending.
Sue Hamilton
Marine Biologist
22 Bryce Crescent
Currie
Midlothian EH14 5LL
0131 449 6209
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[Attachment converted to text. Moderator GBR]
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24th - 26th March 2003
Recently advances in molecular genetics has opened new
avenues of approach to traditional taxonomic studies
based on anatomy and morphology and it is clear that
the power of molecular systematics is being
increasingly appreciated by zoologists and botanist
working with marine flora and fauna.
This course is aimed at people who are engaged in
taxonomic work but who are not familiar with
techniques in molecular systematics. Participants need
not have previous knowledge of the subject as the
course will provide both a basic theoretical grounding
together with instruction in practical techniques.
The course is for anyone who needs to extend their
taxonomic capability beyond traditional morphological
and anatomical approaches. These include taxonomists
working in:
* Museums
* Water authorities
* Government Laboratories
* Consultancies and analytical laboratories
* Research Institutes
* Universities
Programme
Monday 24 th March:
9.00: Arrival in the Department of Biological Sciences
9.15-9.30: Welcome and group organisation
9.30-10.00: Lecture 1: DNA structure and properties,
methods of preparation.
10.15-11.15: Practical 1: Sample preparation
11.15-11.30 Coffee
11.30-11.45: Lecture 2: Safety in the molecular
biology laboratory
11.45-12.45 Lecture 3: PCR methodology
12.45-14.00: Lunch
14.00-16.00: Practical 2: DNA extraction and agarose
gel electrophoresis
16.00-16.15 Refreshments
16.15-16.45: Practical 3: DNA gel electro-phoresis,
documentation, analysis and discussion
16.45-18.00: Practical 4: PCR amplification of DNA
samples
Tuesday 25th March:
9.30-10.00: Practical 5: Gel preparation
10.00-10.15: Practical 6: Sample preparation, loading
gel and electrophoresis
10.15-10.30 Coffee
10.30-11.20: Lecture 4: Sequencing methodology
11.30-12.30: Lecture 5: Molecular systematics:
phylogeny
12.30-14.00: Lunch
14.00-15.00: Lecture 6: Molecular systematics: strain
typing
15.00-16.00: Practical 6: Photo documentation,
sequencer, traces analysis (Part 1).
16.00-16.15: Refreshments
16.15-17.00: Practical 7: Photo documentation,
sequencer, traces analysis (Part 2).
Wednesday 26th March:
9.15-10.30: Bioinformatics session: 1: Analysis of
sequences/Blast
searches, the Ribosomal RNA Database
10.30-10.45 Coffee
10.45-11.45: Bioinformatics session 2: Participants'
requests on specific organisms
11.45-12.30: General discussion
12.30-14.00: Lunch and depart
Lecturers
Dr. Mark Dorris is a molecular biologist with several
years experience in molecular systematics including
DNA analytical techniques such as PCR, SB, RSLP and
DNA sequencing. He currently involved with several
molecular taxonomic studies in the Centre for Marine
Biodiversity and Biotechnology.
Dr. Philip Meaden is a molecular biologist with a
particular interest in the development and analysis of
industrial strains of yeast. He has worked closely
with the brewing industry in the application of DNA
profiling to the characterisation of commercial yeast
strains.
Dr Peter Morris is a plant physiologist by training
and has spent the last 20 years working on various
aspects of plant molecular biology, and has
considerable 'hands-on' experience of DNA extraction,
manipulation and analysis from both plants and marine
organisms.
Professor Fergus Priest is a microbiologist with a
research interest in molecular systematics
particularly of Gram-positive bacteria. His work spans
the range from strain typing and population structures
of bacterial species to the use of gene sequences to
determine generic and species affiliations.
REGISTRATION FORM
Name:
Address:
Dates: Monday 24th March to Wednesday 26th March 2002
I require accommodation for:
23rd-25th March (3 nights)
24th-25th March (2 nights)
Other
Participants may bring their own material for DNA
analysis, subject to its suitability for class work.
Please return form by 10th March 2003 to:
Dr. P.F.Kingston
Centre for Marine Biodiversity and Biotechnology
School of Life Sciences
Heriot-Watt University
Riccarton, Edinburgh, EH14 4AS
Scotland, U.K.
Tel: +131 449 5111
Fax: +131 451 3009
Email: P.F.Kingston at hw.ac.uk
Costs: Tuition and bench fees £300.
Accommodation and meals for three nights £180
Additional nights (B&B only) £40/night
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