Hello Anneliders
The Heriot-Watt Molecular Systematics course due to be held this Easter
has been re-scheduled to take place in June, hoping that this time is
suitable for more people. The details and an application form follow
below. Please reply directly to Paul Kingston and not to me, I'm only
advertising it, not organising it, although I have attended and would
recommend it, especially to complete novices in the subject. Please feel
free to pass this message on to anyone else who you think may be
interested.
Sue
Sue Hamilton, BSc (Hons), MIBiol, CBiol Marine Biologist 22 Bryce
Crescent Currie Midlothian EH14 5LL Tel. +44 (0) 131 449 6209
9th - 11th June 2003
Recently advances in molecular genetics has opened new avenues of
approach to traditional taxonomic studies based on anatomy and
morphology and it is clear that the power of molecular systematics is
being increasingly appreciated by zoologists and botanist working with
marine flora and fauna.
This course is aimed at people who are engaged in taxonomic work but
who are not familiar with techniques in molecular systematics.
Participants need not have previous knowledge of the subject as the
course will provide both a basic theoretical grounding together with
instruction in practical techniques.
The course is for anyone who needs to extend their taxonomic capability
beyond traditional morphological and anatomical approaches. These
include taxonomists working in:
* Museums
* Water authorities
* Government Laboratories
* Consultancies and analytical laboratories
* Research Institutes
* Universities
Programme
Monday 9th June:
9.00: Arrival in the Department of Biological Sciences
9.15-9.30: Welcome and group organisation
9.30-10.00: Lecture 1: DNA structure and properties, methods of preparation.
10.15-11.15: Practical 1: Sample preparation
11.15-11.30 Coffee
11.30-11.45: Lecture 2: Safety in the molecular biology laboratory
11.45-12.45 Lecture 3: PCR methodology
12.45-14.00: Lunch
14.00-16.00: Practical 2: DNA extraction and agarose gel electrophoresis
16.00-16.15 Refreshments
16.15-16.45: Practical 3: DNA gel electro-phoresis, documentation, analysis and discussion
16.45-18.00: Practical 4: PCR amplification of DNA samples
Tuesday 10th June:
9.30-10.00: Practical 5: Gel preparation
10.00-10.15: Practical 6: Sample preparation, loading gel and electrophoresis
10.15-10.30 Coffee
10.30-11.20: Lecture 4: Sequencing methodology
11.30-12.30: Lecture 5: Molecular systematics: phylogeny
12.30-14.00: Lunch
14.00-15.00: Lecture 6: Molecular systematics: strain typing
15.00-16.00: Practical 6: Photo documentation, sequencer, traces analysis (Part 1).
16.00-16.15: Refreshments
16.15-17.00: Practical 7: Photo documentation, sequencer, traces analysis (Part 2).
Wednesday 11th June:
9.15-10.30: Bioinformatics session: 1: Analysis of sequences/Blast
searches, the Ribosomal RNA Database
10.30-10.45 Coffee
10.45-11.45: Bioinformatics session 2: Participants' requests on specific organisms
11.45-12.30: General discussion
12.30-14.00: Lunch and depart
Lecturers
Dr. Mark Dorris is a molecular biologist with several years experience in
molecular systematics including DNA analytical techniques such as PCR,
SB, RSLP and DNA sequencing. He currently involved with several
molecular taxonomic studies in the Centre for Marine Biodiversity and
Biotechnology.
Dr. Philip Meaden is a molecular biologist with a particular interest in the
development and analysis of industrial strains of yeast. He has worked
closely with the brewing industry in the application of DNA profiling to the
characterisation of commercial yeast strains.
Dr Peter Morris is a plant physiologist by training and has spent the last
20 years working on various aspects of plant molecular biology, and has
considerable 'hands-on' experience of DNA extraction, manipulation and
analysis from both plants and marine organisms.
Professor Fergus Priest is a microbiologist with a research interest in
molecular systematics particularly of Gram-positive bacteria. His work
spans the range from strain typing and population structures of bacterial
species to the use of gene sequences to determine generic and species
affiliations.
Registration Form
Please return form by email to: P.F.Kingston at hw.ac.uk or post to:
Dr. P.F.Kingston Centre for Marine Biodiversity and Biotechnology
Department of Biological Sciences Heriot-Watt University Riccarton
Edinburgh, EH14 4AS, Scotland, U.K. Tel: +131 449 5111 Fax: +131
451 3009 Participants may bring their own material for DNA analysis,
subject to its suitability for class work.
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