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Molecular Systematics Course

Sue Hamilton sue.hamilton at totalise.co.uk
Sat Mar 22 17:35:24 EST 2003

Hello Anneliders   

The Heriot-Watt Molecular Systematics course due to be held this Easter 
has been re-scheduled to take place in June, hoping that this time is 
suitable for more people.  The details and an application form follow 
below.  Please reply directly to Paul Kingston and not to me, I'm only 
advertising it, not organising it, although I have attended and would 
recommend it, especially to complete novices in the subject.  Please feel 
free to pass this message on to anyone else who you think may be 


Sue Hamilton, BSc (Hons), MIBiol, CBiol Marine Biologist 22 Bryce 
Crescent Currie Midlothian EH14 5LL Tel. +44 (0) 131 449 6209  

9th - 11th June 2003

Recently advances in molecular genetics has opened new avenues of 
approach to traditional taxonomic studies based on anatomy and 
morphology and it is clear that the power of molecular systematics is 
being increasingly appreciated by zoologists and botanist working with 
marine flora and fauna.  

This course is aimed at people who are engaged in taxonomic work but 
who are not familiar with techniques in molecular systematics. 
Participants need not have previous knowledge of the subject as the 
course will provide both a basic theoretical grounding together with 
instruction in practical techniques.  

The course is for anyone who needs to extend their taxonomic capability 
beyond traditional morphological and anatomical approaches. These 
include taxonomists working in:  

* Museums 
* Water authorities
* Government Laboratories
* Consultancies and analytical laboratories
* Research Institutes
* Universities


Monday 9th June:

9.00:	Arrival in the Department of Biological Sciences
9.15-9.30:		Welcome and group organisation
9.30-10.00:	Lecture 1: DNA structure and properties, methods of preparation.
 10.15-11.15:	Practical 1: Sample preparation
11.15-11.30	Coffee
11.30-11.45:	Lecture 2: Safety in the molecular biology laboratory
11.45-12.45	Lecture 3: PCR methodology
12.45-14.00:	Lunch
14.00-16.00:	Practical 2: DNA extraction and agarose gel electrophoresis
16.00-16.15	Refreshments
16.15-16.45:	Practical 3: DNA gel electro-phoresis, documentation, analysis and discussion
16.45-18.00:	Practical 4: PCR amplification of DNA samples
Tuesday 10th June:

9.30-10.00:	Practical 5: Gel preparation
10.00-10.15:	Practical 6: Sample preparation, loading gel and electrophoresis
10.15-10.30	Coffee
10.30-11.20:	Lecture 4: Sequencing methodology
11.30-12.30:	Lecture 5: Molecular systematics: phylogeny
12.30-14.00:	Lunch

14.00-15.00:	Lecture 6: Molecular systematics: strain typing
15.00-16.00:	Practical 6: Photo documentation, sequencer, traces analysis (Part 1).
16.00-16.15:	Refreshments
16.15-17.00:	Practical 7: Photo documentation, sequencer, traces analysis (Part 2).

Wednesday 11th June:

9.15-10.30:	Bioinformatics session: 1: Analysis of sequences/Blast 
searches, the Ribosomal RNA Database

10.30-10.45	Coffee

10.45-11.45:	Bioinformatics session 2: Participants' requests on specific organisms
11.45-12.30:	General discussion 

12.30-14.00:	Lunch and depart


Dr. Mark Dorris is a molecular biologist  with several years experience in 
molecular systematics including DNA analytical techniques such as PCR, 
SB, RSLP and DNA sequencing. He currently involved with several 
molecular taxonomic studies in the Centre for Marine Biodiversity and 

Dr. Philip Meaden is a molecular biologist with a particular interest in the 
development and analysis of industrial strains of yeast.  He has worked 
closely with the brewing industry in the application of DNA profiling to the 
characterisation of commercial yeast strains.  

Dr Peter Morris is a plant physiologist by training and has spent the last 
20 years working on various aspects of plant molecular biology, and has 
considerable 'hands-on' experience of DNA extraction, manipulation and 
analysis from both plants and marine organisms.  

Professor Fergus Priest is a microbiologist with a research interest in 
molecular systematics particularly of Gram-positive bacteria. His work 
spans the range from strain typing and population structures of bacterial 
species to the use of gene sequences to determine generic and species 

Registration Form

Please return form by email to:	 P.F.Kingston at hw.ac.uk or post to: 
Dr. P.F.Kingston Centre for Marine Biodiversity and Biotechnology 
Department of Biological Sciences Heriot-Watt University Riccarton 
Edinburgh, EH14 4AS, Scotland, U.K. Tel: +131 449 5111   Fax: +131 
451 3009 Participants may bring their own material for DNA analysis, 
subject to its suitability for class work.  

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