[Annelida] SEM question

[J. Kirk Fitzhugh]

I use HMDS on a regular basis with excellent results.  It is especially 
great if you are working with very small specimens or parts of specimens, 
since there is no risk of losing them. I can send you the protocol I use if 
you wish.

Kirk

At 05:07 PM 2/15/2006 +0000, you wrote:
>Aloha,
>
>I was getting geared up to do some SEM this week, and wondered if anyone
>has had any experience with the CPD alternative Hexamethyldisilazane
>(HMDS)? The technicians on campus are split on the subject and I have
>heard testimonials from both sides. I have not seen it used in many
>articles, but if there are no major drawbacks I thought it would be a nice
>technique to pick up.
>
>I would appreciate your thoughts on the method.
>
>Cheers,
>
>chip
>
>
>*************************************
>
>Brendan "Chip" Barrett
>Martin Ryan Institue
>National University of Ireland Galway,
>Galway, Co. Galway, Ireland
>
>*************************************
>
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-----------------------------------------------------
J. Kirk Fitzhugh, Ph.D.
Curator of Polychaetes
Invertebrate Zoology Section
Research & Collections Branch
Los Angeles County Museum of Natural History
900 Exposition Blvd
Los Angeles CA 90007

Phone:   213-763-3233
FAX:       213-746-2999
e-mail:  kfitzhug at nhm.org
http://www.nhm.org/research/annelida/staff.html
http://www.nhm.org/research/annelida/index.html
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