From g.read from niwa.co.nz Mon Mar 3 15:33:19 2008 From: g.read from niwa.co.nz (Geoff Read) Date: Mon Mar 3 15:35:48 2008 Subject: [Annelida] Gulf of Mexico books Re: Ubelacher & Johnson free online Message-ID: <47CD175F.8045.00D5.0@niwa.co.nz> Hi, Time to update (thanks to Helmut Zibrowius) that the "Taxonomic guide to the polychaetes of the northern Gulf of Mexico" series are still online. This is now the general search form: http://www.mrm.mms.gov:8765/query.html This the MMS site: http://www.mrm.mms.gov This the entry point to all environmental publications: http://www.gomr.mms.gov/homepg/espis/espisfront.asp The url providing a direct link to all the volumes that some (including me) may have saved way back no longer exists. Geoff >>> On 14/03/2003 at 8:19 a.m., Robert S Carney wrote: > Minerals Management Service (MMS) is making all its completed projects > available for pdf download. This includes the 7 vols for Gulf of Mexico > polychaetes. > > Go to http://mmspub.mms.gov:81/search.html > > For tile enter Taxonomic > For region select Gulf of Mexico > > This should return all 7 volumes. Download as per your browser. > > These were scanned in, and in conversion to pdf format there has been > some > corruption in figures and unusual text. The downloads are, however, 99.5% > correct and very useful. > > Bob Carney > LSU -- Geoff Read http://www.annelida.net/ http://www.niwascience.co.nz/ncabb/ From g.read from niwa.co.nz Sat Mar 8 03:00:05 2008 From: g.read from niwa.co.nz (Geoff Read) Date: Sat Mar 8 03:03:33 2008 Subject: [Annelida] Farming a Diopatra in Oz Message-ID: <47D2FE55.8045.00D5.0@niwa.co.nz> Hi all, Here is a marine worm farm item from an Australian aquaculture magazine that some might find interesting. O'Sullivan, D. 2007: Farmed tube worms by the millions. Austasia Aquaculture 21: 3-9. (December) I've attached the first page only. The article is online via link below, but I'm not sure that it's open access (we subscribe). See also: Safarik, M.; Redden, A. M. ; Schreider, M. J. 2006: Density-dependent growth of the polychaete Diopatra aciculata. Scientia Marina 70: 337-341. The Magazine: http://www.austasiaaquaculture.com.au/ The farm: http://www.aquabait.com.au Cheers, Geoff -- Geoff Read http://www.annelida.net/ http://www.niwascience.co.nz/ncabb/ -------------- next part -------------- A non-text attachment was scrubbed... Name: OSullivanIntro.pdf Type: application/pdf Size: 241201 bytes Desc: not available Url : http://www.bio.net/bionet/mm/annelida/attachments/20080308/89e12d7d/OSullivanIntro.pdf From gemma.robinson from nhm.ac.uk Tue Mar 11 09:56:47 2008 From: gemma.robinson from nhm.ac.uk (Gemma Robinson) Date: Tue Mar 11 13:43:32 2008 Subject: [Annelida] SYNTHESYS Funding available- Final Call for proposal Message-ID: <15CF608D129D504FA6A540E374F1463DA1CBD1@HOMER.nhm.ac.uk> Dear Christos, Please can you circulate the below information to members of the MedOBIS Discussion Group (Annelida). Many thanks Gemma ------------------------------------------------- Dear Scientist, Final Call Deadline; 28th March 2008 The SYNTHESYS Office is pleased to announce the Eighth and final call for proposals under the European Commission's FPVI European-funded Integrated Infrastructure Initiative. SYNTHESYS Project funding is available to provide scientists (Users) based in European Member, Associate and Candidate States to undertake short visits to utilize the infrastructure (namely the collections, staff expertise and analytical facilities) at one of the 20 partner institutions (see full list below) for the purposes of their research. The 20 partner institutions are organised into 11 national Taxonomic Facilities (TAFs). TAF Users will be hosted by a TAF staff member. The 11 TAF institutions represent an unparalleled resource for taxonomic research offering: Collections amounting to over 337 million natural history specimens, including 3.3 million type specimens, internationally renowned taxonomic and systematic skill base, and chemical analysis, molecular and imaging facilities. Proposals will be welcomed from high calibre researchers seeking access for short-term visits. SYNTHESYS is able to meet the Users' costs for: ? Research costs* ? International travel ? Local accommodation whilst based at the TAF ? A per diem to contribute towards living costs * Research related costs including: bench fees and consumables (including molecular biology at some TAFs). See www.synthesys.info for more information or contact synthesys@nhm.ac.uk This is the last opportunity for SYNTHESYS funding, so please circulate this information amongst your colleagues. Best wishes Gemma Gemma Robinson SYNTHESYS Administrator Natural History Museum Cromwell Road London SW7 5BD SYNTHESYS Partners: GB-TAF Natural History Museum, Royal Botanic Gardens, Kew, Royal Botanic Garden Edinburgh. ES-TAF Museo Nacional de Ciencias Naturales, Real Jard?n Bot?nico Naturales. DK-TAF The Natural History Museum of Denmark. FR-TAF Museum National d'Histoire Naturelle. SE-TAF Naturhistoriska Riksmuseet. NL-TAF University van Amsterdam, Nationaal Herbarium Nederland, Centraalbureau voor Schimmelcultures, National Natural History Museum Naturalis. DE-TAF Museum fur Naturkunde, Botanischer Garten und Botanisches Museum. AT-TAF Naturhistorisches Museum, Wien. HU-TAF Hungarian Natural History Museum. PL-TAF Museum and Institute of Zoology, Polish Academy of Sciences. BE-TAF Royal Belgian Institute of Natural Sciences, Koninklijik Museum voor Midden-Afrika, National Botanic Garden of Belgium. 1 The SYNTHESYS Partners are inviting applications from researchers based in the Member States of the EU: Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, United Kingdom plus the Associated Countries of the EU: Switzerland, Iceland, Israel, Liechtenstein, Norway and Candidate Countries of the EU: Croatia, FYR Macedonia, Republic of Serbia and Turkey. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/annelida/attachments/20080311/8aa848e5/attachment.html From dimitricostacg from gmail.com Wed Mar 12 09:34:55 2008 From: dimitricostacg from gmail.com (=?ISO-8859-1?Q?Dim=EDtri_de_Ara=FAjo_Costa?=) Date: Wed Mar 12 13:45:00 2008 Subject: [Annelida] Papers Message-ID: <2c3e659f0803120734y2613dd49sca716f2a7186534b@mail.gmail.com> I would greatly appreciate a reprint these papers: Hickok, J.F. and Davenport, D. 1957. Further studies in the behavior of commensal polychaetes. *Biological Bulletin*. 113:397-406. Stewart, W.C, 1970. A study of the nature of the attractant emitted by the asteroid hosts of the commensal polychaete, *Ophiodromus puggetensis. Dissertation, University of California Santa Barbara*, 95 pp. -- Dim?tri de Ara?jo Costa Laborat?rio de Invertebrados Paulo Young Departamento de Sistem?tica e Ecologia - DSE Centro de Ci?ncias Exatas e da Natureza - CCEN Universidade Federal da Para?ba Jo?o Pessoa - Para?ba CEP: 58059-900 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/annelida/attachments/20080312/5e17abc5/attachment.html From halankm from auburn.edu Wed Mar 12 14:46:44 2008 From: halankm from auburn.edu (Ken Halanych) Date: Wed Mar 12 14:51:31 2008 Subject: [Annelida] Papers In-Reply-To: <2c3e659f0803120734y2613dd49sca716f2a7186534b@mail.gmail.com> References: <2c3e659f0803120734y2613dd49sca716f2a7186534b@mail.gmail.com> Message-ID: <2BC998E9-FCE1-40D2-8DEA-587422930A38@auburn.edu> Dimitri All of the old Biological Bulletin issues are available for pdf down load at: http://www.biolbull.org/ The paper you seek is at http://www.biolbull.org/cgi/reprint/113/3/397 On Mar 12, 2008, at 9:34 AM, Dim?tri de Ara?jo Costa wrote: > Hickok, J.F. and Davenport, D. 1957. Further studies in the > behavior of commensal polychaetes. Biological Bulletin. 113:397-406. ************************************ Kenneth M. Halanych Alumni Professor Marine Biology Coordinator Life Sciences Department Rouse 101 Auburn University Auburn, AL 36849 http://gump.auburn.edu/halanych/lab/index.html Phone: (334)-844-3222 Ken's Fax: (334)-844-2333 Biology Fax (334)-844-1645 e-mail: ken@auburn.edu ************************************ -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/annelida/attachments/20080312/49668464/attachment.html From paulo.paiva from gmail.com Thu Mar 13 05:46:20 2008 From: paulo.paiva from gmail.com (Paulo Paiva) Date: Thu Mar 13 13:47:44 2008 Subject: [Annelida] Papers In-Reply-To: <2BC998E9-FCE1-40D2-8DEA-587422930A38@auburn.edu> References: <2c3e659f0803120734y2613dd49sca716f2a7186534b@mail.gmail.com> <2BC998E9-FCE1-40D2-8DEA-587422930A38@auburn.edu> Message-ID: <1e0b04ad0803130346m22b2e962l8f1fa5880c3c8fdc@mail.gmail.com> Does anyone have a pdf copy of the original description of Chaetopterus variopedatus (Renier, S.A. 1804. Prospetto della classe dei Vermi pp. XV–XXVII. Padua) ? Thanks in advance, Paulo Paiva -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/annelida/attachments/20080313/b12ef3a0/attachment.html From g.read from niwa.co.nz Thu Mar 13 16:38:43 2008 From: g.read from niwa.co.nz (Geoff Read) Date: Thu Mar 13 16:51:25 2008 Subject: Renier 1804 Re: [Annelida] Papers In-Reply-To: <1e0b04ad0803130346m22b2e962l8f1fa5880c3c8fdc@mail.gmail.com> References: <2c3e659f0803120734y2613dd49sca716f2a7186534b@mail.gmail.com> <2BC998E9-FCE1-40D2-8DEA-587422930A38@auburn.edu> <1e0b04ad0803130346m22b2e962l8f1fa5880c3c8fdc@mail.gmail.com> Message-ID: <47DA55B3.8045.00D5.0@niwa.co.nz> >>> On 13/03/2008 at 11:46 p.m., "Paulo Paiva" wrote: > Does anyone have a pdf copy of the original description of Chaetopterus > variopedatus (Renier, S.A. 1804. Prospetto della classe dei Vermi pp. > XV?XXVII. Padua) ? Paulo & all, I've never seen it, but note that Renier, 1804, an incomplete work, is rejected for nomenclatural purposes (ICZN Opinion 316). Some names in other Phyla (eg, Nemertea) have been reinstated since, but variopedatus has not been I believe. Someone with access will be able to say if the names listed are given diagnoses. But I would suspect not (without checking why it's rejected). So, other than for nomenclatural history, it's probably not much use to us. Second, I'd just like to mention that the placing of this work in Zool. Jahrb. Syst., Jena 64: 41?110, as is circulating in some recent-ish publications, one very prominent, is incorrect. That journal citation refers to a paper written in 1933 by Hans REIMERS on Sphaerodorum. Someone had a dyslexic moment? Cheers, Geoff -- Geoff Read http://www.annelida.net/ http://www.niwascience.co.nz/ncabb/ From g.read from niwa.co.nz Fri Mar 14 02:58:22 2008 From: g.read from niwa.co.nz (Geoff Read) Date: Fri Mar 14 03:04:28 2008 Subject: [Annelida] Smithsonian digital depository / Pink book / Zootaxa Message-ID: <47DAE6EE.8045.00D5.0@niwa.co.nz> Hi all, I continue the theme of discovering digital content. The Smithsonian digital depository now includes quite a number of Kristian Fauchald and Marian Pettibone papers, http://si-pddr.si.edu/dspace/ notably a searchable copy of Kristian's indispensable 'pink book' concise summary of all polychaete knowledge up to 1977. I guess we haven't heard about it previously because it (they?) seems to have only became available during Feb 2008. http://hdl.handle.net/10088/3435 If you encounter seagrasses in your work, then this is available: Phillips, Ronald C, and Ernani G. Meriez. Seagrasses. Smithsonian Contributions to the Marine Sciences, number 34, 104 pages, 4 tables, 57 figures, 39 maps, 1988.-This work presents general and current information on seagrass ecology, physiology, biology, distribution and evolution. Additionally, all known taxa of seagrasses are keyed to recognized species. Forty-eight species are described and illustrated, with accompanying maps to indicate their world distribution. http://hdl.handle.net/10088/1131 Finally I also noticed that Zootaxa is now offering taxon-level annual subscriptions. Prices reflect the number of papers likely to be produced, with a subscription to Annelida at usd 52 / yr, http://www.mapress.com/zootaxa/support/subannual2.html Cheers, Geoff -- Geoff Read http://www.annelida.net/ http://www.niwascience.co.nz/ncabb/ From FAUCHALD from si.edu Fri Mar 14 09:44:52 2008 From: FAUCHALD from si.edu (Fauchald, Kristian) Date: Fri Mar 14 15:06:56 2008 Subject: [Annelida] Smithsonian digital depository / Pink book / Zootaxa In-Reply-To: <47DAE6EE.8045.00D5.0@niwa.co.nz> References: <47DAE6EE.8045.00D5.0@niwa.co.nz> Message-ID: <707264088F25964398B036DDCEB1C0F00445832B@SI-ECL01.US.SINET.SI.EDU> Thank you Geoff for pointing this out; we are coming "on-line" in bits and pieces and I never know when anything is going to happen. Let me put it this way, please check first to see if any paper you need, is available on the web. Then, if you cannot find it, send me a message, since we do have more papers scanned, if not in the proper location for being downloaded by non-staff members. Kristian Fd -----Original Message----- From: annelida-bounces@oat.bio.indiana.edu [mailto:annelida-bounces@oat.bio.indiana.edu] On Behalf Of Geoff Read Sent: Friday, March 14, 2008 3:58 AM To: annelida@magpie.bio.indiana.edu Subject: [Annelida] Smithsonian digital depository / Pink book / Zootaxa Hi all, I continue the theme of discovering digital content. The Smithsonian digital depository now includes quite a number of Kristian Fauchald and Marian Pettibone papers, http://si-pddr.si.edu/dspace/ notably a searchable copy of Kristian's indispensable 'pink book' concise summary of all polychaete knowledge up to 1977. I guess we haven't heard about it previously because it (they?) seems to have only became available during Feb 2008. http://hdl.handle.net/10088/3435 If you encounter seagrasses in your work, then this is available: Phillips, Ronald C, and Ernani G. Meriez. Seagrasses. Smithsonian Contributions to the Marine Sciences, number 34, 104 pages, 4 tables, 57 figures, 39 maps, 1988.-This work presents general and current information on seagrass ecology, physiology, biology, distribution and evolution. Additionally, all known taxa of seagrasses are keyed to recognized species. Forty-eight species are described and illustrated, with accompanying maps to indicate their world distribution. http://hdl.handle.net/10088/1131 Finally I also noticed that Zootaxa is now offering taxon-level annual subscriptions. Prices reflect the number of papers likely to be produced, with a subscription to Annelida at usd 52 / yr, http://www.mapress.com/zootaxa/support/subannual2.html Cheers, Geoff -- Geoff Read http://www.annelida.net/ http://www.niwascience.co.nz/ncabb/ _______________________________________________ Annelida mailing list Post: Annelida@net.bio.net Help/archive: http://www.bio.net/biomail/listinfo/annelida Resources: http://www.annelida.net From viviannesw from yahoo.com Thu Mar 13 17:54:09 2008 From: viviannesw from yahoo.com (vivianne solis) Date: Fri Mar 14 15:07:52 2008 Subject: [Annelida] address required... Message-ID: <5537.90781.qm@web36411.mail.mud.yahoo.com> Dear Colleagues: Would anyone know where I can find the email address of Brigitte Hilbig? The addresses officialy listed here or elsewhere are not working. Maybe you are reading this Brigitte?? I take this opportunity to wish everyone a very successful year Vivianne --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/annelida/attachments/20080313/40500def/attachment.html From viviannesw from yahoo.com Mon Mar 17 12:43:48 2008 From: viviannesw from yahoo.com (vivianne solis) Date: Tue Mar 18 13:47:07 2008 Subject: [Annelida] Brgitte' Hilbig address Message-ID: <320213.72738.qm@web36405.mail.mud.yahoo.com> Hi colleagues! Thanks a lot to all that have taken the time to forward me the addresses they had for Brigitte Hilbig, I really appreciate it. Unfortunately, those were the ones I had already tried unsuccesfuly, I re-take them here so you know all those that do not work anymore (at least for me... the mails are returned) bhilbig@senckenberg.de Bhilbig@zoologie.uni-hamburg.de brigitte.hilbig@ruhr-uni-bochum.de Seems we all had the same info, I don't know where she can be found. In fact, the reason I am looking for her is that we need to locate the holotype of Pisione galapagoensis Westheide 1974. That info would be very much appreciated if she cannot provide it. Sorry for the trouble, and thanks in advance for your feedback ... Wormly Vivianne --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/annelida/attachments/20080317/425b2e24/attachment.html From g.read from niwa.co.nz Tue Mar 18 14:40:57 2008 From: g.read from niwa.co.nz (Geoff Read) Date: Tue Mar 18 14:43:50 2008 Subject: [Annelida] Brgitte' Hilbig address In-Reply-To: <320213.72738.qm@web36405.mail.mud.yahoo.com> References: <320213.72738.qm@web36405.mail.mud.yahoo.com> Message-ID: <47E0D197.8045.00D5.0@niwa.co.nz> Vivianne & all, Re Brigitte - AFAIK it is still bhilbig@senckenberg.de. Perhaps there is a temporary mail server problem at Senckenberg. I tested mail to people at Senckenberg via a web test utility just now and it failed. Brigitte's (aka Brigitte Ebbe) contact phones (if she is there and not at sea) are supposedly: Telephone: +49 (228) 9122-285 Fax: +49 (228) 9122-202 Those are phones in Bonn (not Frankfurt) web page: http://www.senckenberg.de/root/index.php?page_id=2815 Senckenberg institute phones (in Frankfurt) are: Phone: +49 (0)69/7542-0 Fax: +49 (0)69/746238 Cheers, Geoff >>> On 18/03/2008 at 6:43 a.m., vivianne solis wrote: > Hi colleagues! > > Thanks a lot to all that have taken the time to forward me the addresses > they had for Brigitte Hilbig, I really appreciate it. > > Unfortunately, those were the ones I had already tried unsuccesfuly, I > re-take them here so you know all those that do not work anymore (at least > for me... the mails are returned) > > bhilbig@senckenberg.de > Bhilbig@zoologie.uni-hamburg.de > brigitte.hilbig@ruhr-uni-bochum.de > > Seems we all had the same info, I don't know where she can be found. > > In fact, the reason I am looking for her is that we need to locate the > holotype of Pisione galapagoensis Westheide 1974. That info would be very > much appreciated if she cannot provide it. > > Sorry for the trouble, and thanks in advance for your feedback ... > Wormly > Vivianne -- Geoff Read http://www.annelida.net/ http://www.niwascience.co.nz/ncabb/ From Stanislas.Dubois from ifremer.fr Wed Mar 19 10:27:13 2008 From: Stanislas.Dubois from ifremer.fr (Stanislas DUBOIS) Date: Wed Mar 19 13:59:06 2008 Subject: [Annelida] formol vs RCL2 Message-ID: <47E130D1.3000302@ifremer.fr> Dear Polychaete lovers, For safety and health reasons, formaldehyde (formol) will be soon prohibited (at least in Europe .. I'm not sure for other countries). That might be problematic for specimen preservation since, as many of you I guess, I used to fix collected polychaetes from benthic samples in formaldehyde. I just learned that RCL2 might be of great interest to substitute for formol. Does anyone already experience this surrogate formol or does anyone has any information to share with me, as far as fixation quality of tissues for IDs (I'm not talking of doing any molecular analysis), preservation of pigments etc.... Thanks for your inputs. Stan. -- ______________________________________________________ Stanislas DUBOIS (PhD) Laboratoire DYNECO / Ecologie Benthique IFREMER - French Research Institut for Exploitation of the Sea Technopole de Brest-Iroise B.P. 70 - 29280 Plouzan? - FRANCE Tel: (33) 02 98 22 49 18 Fax: (33) 02 98 22 45 48 email: sdubois@ifremer.fr From Anna.Murray from austmus.gov.au Wed Mar 19 17:47:52 2008 From: Anna.Murray from austmus.gov.au (Anna Murray) Date: Wed Mar 19 17:55:58 2008 Subject: [Annelida] Polychaete Post-Doc oppportunity at the AM Message-ID: Pat Hutchings would like to inform all polychaete-workers of the following opportunity: POSTDOCTORAL FELLOW - POLYCHAETA. Scientific Officer, Grade 2. Temporary full-time.Position No. TMP596/08. Total remuneration package valued at up to $78,951 p.a, including salary ($64,827- $71,546), employers contribution to superannuation and annual leave loading. Responsible for systematic and biogeographic research on the Sabellida clade, describing new taxa and undertaking phylogenetic studies using morphological and molecular data. within the Siboglinids, Oweniids and Sabellids. Selection Criteria: PhD degree in marine science with detailed taxonomic knowledge of Sabellida and publishing experience in the group. Experience in molecular biology, scanning electron microscopy, and developing interactive keys. An ability to undertake fieldwork. Well developed written and oral communication skills. Experience in the use of analytical computer programs. Ability to undertake independent research. Job Notes: This is a postdoctoral fellowship opportunity, jointly funded by the Australian Biological Resources Study (ABRS) and the Australian Museum. Additional funds will be available for laboratory, travel and field expenses. The intention of this Fellowship is to build the Australian taxonomic capacity within key groups. This is a temporary position with appointment/employment under Section 24, 27 or 86 of the Public Sector Employment and Management 2002 for a period of up to three (3) years Inquiries: Dr Brian Lassig on (02) 9320 6297 or email brian.lassig@austmus.gov.au Closing date: 30 April 2008 Please visit Australian Museum - Positions Vacant for full job details including selection criteria, inquiries contact and the position description. Anna Murray Marine Invertebrates Australian Museum 6 College Street Sydney NSW 2010 Australia t 61 2 9320 6248 f 61 2 9320 6042 www.australianmuseum.net.au Inspiring the exploration of nature and cultures The Australian Museum. The views in this email are those of the user and do not necessarily reflect the views of the Australian Museum. The information contained in this email message and any accompanying files is or may be confidential and is for the intended recipient only. If you are not the intended recipient, any use, dissemination, reliance, forwarding, printing or copying of this email or any attached files is unauthorised. If you are not the intended recipient, please delete it and notify the sender. The Australian Museum does not guarantee the accuracy of any information contained in this e-mail or attached files. As Internet communications are not secure, the Australian Museum does not accept legal responsibility for the contents of this message or attached files. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/annelida/attachments/20080320/24e76a26/attachment.html From g.read from niwa.co.nz Wed Mar 19 21:01:32 2008 From: g.read from niwa.co.nz (Geoff Read) Date: Wed Mar 19 21:10:53 2008 Subject: [Annelida] formol vs RCL2 In-Reply-To: <47E130D1.3000302@ifremer.fr> References: <47E130D1.3000302@ifremer.fr> Message-ID: <47E27C4B.8045.00D5.0@niwa.co.nz> >>> On 20/03/2008 at 4:27 a.m., Stanislas DUBOIS > For safety and health reasons, formaldehyde (formol) will be soon > prohibited (at least in Europe .. I'm not sure for other countries). > That might be problematic for specimen preservation since, as many of > you I guess, I used to fix collected polychaetes from benthic samples in > formaldehyde. I just learned that RCL2 might be of great interest to > substitute for formol. Does anyone already experience this surrogate > formol or does anyone has any information to share with me, as far as > fixation quality of tissues for IDs (I'm not talking of doing any > molecular analysis), preservation of pigments etc.... Thanks for the news Stan. My guess is that RCL2 has yet to spread far in the medical world, and even less into general use. The formulation is not public but contains acetic acid (vinegar). Perhaps someone can find out how much more expensive it is compared to formalin, bearing in mind that it's the medical/pathology market that they're selling it into. Those interested can check the company web page: http://www.alphelys.com/site/us/pFF_FixateurSansFormol.htm The paper of 2006 by Delfour et al that they cite, is available full text at pubmed "RCL2, a New Fixative, Preserves Morphology and Nucleic Acid Integrity in Paraffin-Embedded Breast Carcinoma and Microdissected Breast Tumor Cells" http://www.pubmedcentral.nih.gov/ just search for rcl2. Yes, there is a general understandable reluctance of field workers to use formalin these days. I dislike working with it also. But, I find the usual substitute - ethanol - can result in rigid brittle specimens that are very inferior to those formalin-fixed for morphology and identification, or in disintegrating rubbish in bulk, sediment-laden samples. Geoff -- Geoff Read http://www.annelida.net/ http://www.niwascience.co.nz/ncabb/ From A.J.van_Dam from lumc.nl Thu Mar 20 06:36:42 2008 From: A.J.van_Dam from lumc.nl (Andries J. van Dam) Date: Thu Mar 20 14:30:35 2008 Subject: [Annelida] formol vs RCL2 In-Reply-To: <67B36D023E7A744BB4C9C83DCA800150031E550D@NHBEXC01.mus-nature.ca> Message-ID: Dear All, My first thoughts about RCL2, a possible substitute for formol that seems to be successfully applied in medical histopathology. It seems that looking at the patent that RCL2 is 70% EtOH with the addition of acetic acid (likely added for better/stronger fixation) and trehalose, a disaccharide that is part of plants and involved in the protection against dehydration. The interesting thing about this fixative/preservative is that the acidic alcohol its dehydration properties are possibly neutralized by the trehalose which apparently makes the alcohol mixture not only usable for preserving/analysing DNA and (functional) proteins but also provide good morphology, which is a problem with plain ethanol 75% (shrinkage and cell collapse). I am curious about the pH and its stability. Also has to be remarked that it is like ethanol still a hazardous compound with regard to flammability. Other alternatives for formalin might be found in DMDM-hydantoin a safe alternative for formalin used primarily in shampoos and hand soaps. More info about DMDMH: van Dam, Andries J. 2003. DMDM-Hydantoin: The promising Result of a Search for an Alternative in Fluid Preservation of Biological Specimens. Collection Forum, 18(1-2):104-115. Molin, Dani?l G.M.?and Andries J. Van?Dam. 2006. A Preservation Method Supporting Multipurpose Analysis of Long-stored Samples. Cell Preservation Technology. March 1, 2006, 4(1): 46-50. -- Andries J. van Dam, conservator Co-ordinator ICOM-CC Working Group Natural History Collections http://icom-cc.org/WG/NaturalHistoryCollections Administrator WG-interactions, the official forum site of ICOM-CC: http://icom-cc-wg.phpbb24.com (launch January 2008) New address: Museum of Anatomy Leiden University Medical Center Postal zone T7-P P.O. Box 9600 2300 RC Leiden The Netherlands tel: +31 (0)71 526 9581 fax: +31 (0)71 526 8275 E-mail: A.J.van_Dam@lumc.nl Visiting address: Hippocratespad 21, building 3 From: annelida-bounces@oat.bio.indiana.edu on behalf of Geoff Read Sent: Wed 3/19/2008 10:01 PM To: annelida mailing list Subject: Re: [Annelida] formol vs RCL2 >>> On 20/03/2008 at 4:27 a.m., Stanislas DUBOIS > For safety and health reasons, formaldehyde (formol) will be soon > prohibited (at least in Europe .. I'm not sure for other countries). > That might be problematic for specimen preservation since, as many of > you I guess, I used to fix collected polychaetes from benthic samples in > formaldehyde. I just learned that RCL2 might be of great interest to > substitute for formol. Does anyone already experience this surrogate > formol or does anyone has any information to share with me, as far as > fixation quality of tissues for IDs (I'm not talking of doing any > molecular analysis), preservation of pigments etc.... Thanks for the news Stan. My guess is that RCL2 has yet to spread far in the medical world, and even less into general use. The formulation is not public but contains acetic acid (vinegar). Perhaps someone can find out how much more expensive it is compared to formalin, bearing in mind that it's the medical/pathology market that they're selling it into. Those interested can check the company web page: http://www.alphelys.com/site/us/pFF_FixateurSansFormol.htm The paper of 2006 by Delfour et al that they cite, is available full text at pubmed "RCL2, a New Fixative, Preserves Morphology and Nucleic Acid Integrity in Paraffin-Embedded Breast Carcinoma and Microdissected Breast Tumor Cells" http://www.pubmedcentral.nih.gov/ just search for rcl2. Yes, there is a general understandable reluctance of field workers to use formalin these days. I dislike working with it also. But, I find the usual substitute - ethanol - can result in rigid brittle specimens that are very inferior to those formalin-fixed for morphology and identification, or in disintegrating rubbish in bulk, sediment-laden samples. Geoff -- Geoff Read http://www.annelida.net/ http://www.niwascience.co.nz/ncabb/ _______________________________________________ Annelida mailing list Post: Annelida@net.bio.net Help/archive: http://www.bio.net/biomail/listinfo/annelida Resources: http://www.annelida.net -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/annelida/attachments/20080320/fb32027c/attachment.html From Christopher.B.Floyd from usace.army.mil Thu Mar 20 16:45:57 2008 From: Christopher.B.Floyd from usace.army.mil (Floyd, Christopher B POA) Date: Thu Mar 20 17:47:04 2008 Subject: [Annelida] formol vs RCL2 In-Reply-To: <47E27C4B.8045.00D5.0@niwa.co.nz> References: <47E130D1.3000302@ifremer.fr> <47E27C4B.8045.00D5.0@niwa.co.nz> Message-ID: <3C0A8F722A1D704FB428E4D5B593523A07EC5694@poa-ml1anc.poa.ds.usace.army.mil> Another histology product, probably very similar if not identical to RCL2, is 'NOTOXhisto' by the US company at the link: http://www.scientificdevice.com/product_pages/notox.htm I bought some of this product to use on field specimens last year to try as a last-minute substitute, when I found that acquiring a new supply of formalin was going to be prohibitively difficult. It seemed to work adequately for our purposes, at least in the short term... but then we don't do fine-anatomy work, and my old-school biologist co-worker is skeptical of it. I was wondering if anybody else out there had tried using NOTOXhisto on whole specimens. thanks much, Chris Floyd US Army Corps of Engineers Anchorage, Alaska -----Original Message----- From: annelida-bounces@oat.bio.indiana.edu [mailto:annelida-bounces@oat.bio.indiana.edu] On Behalf Of Geoff Read Sent: Wednesday, March 19, 2008 6:02 PM To: annelida mailing list Subject: Re: [Annelida] formol vs RCL2 >>> On 20/03/2008 at 4:27 a.m., Stanislas DUBOIS >>> > For safety and health reasons, formaldehyde (formol) will be soon > prohibited (at least in Europe .. I'm not sure for other countries). > That might be problematic for specimen preservation since, as many of > you I guess, I used to fix collected polychaetes from benthic samples > in formaldehyde. I just learned that RCL2 might be of great interest > to substitute for formol. Does anyone already experience this > surrogate formol or does anyone has any information to share with me, > as far as fixation quality of tissues for IDs (I'm not talking of > doing any molecular analysis), preservation of pigments etc.... Thanks for the news Stan. My guess is that RCL2 has yet to spread far in the medical world, and even less into general use. The formulation is not public but contains acetic acid (vinegar). Perhaps someone can find out how much more expensive it is compared to formalin, bearing in mind that it's the medical/pathology market that they're selling it into. Those interested can check the company web page: http://www.alphelys.com/site/us/pFF_FixateurSansFormol.htm The paper of 2006 by Delfour et al that they cite, is available full text at pubmed "RCL2, a New Fixative, Preserves Morphology and Nucleic Acid Integrity in Paraffin-Embedded Breast Carcinoma and Microdissected Breast Tumor Cells" http://www.pubmedcentral.nih.gov/ just search for rcl2. Yes, there is a general understandable reluctance of field workers to use formalin these days. I dislike working with it also. But, I find the usual substitute - ethanol - can result in rigid brittle specimens that are very inferior to those formalin-fixed for morphology and identification, or in disintegrating rubbish in bulk, sediment-laden samples. Geoff -- Geoff Read http://www.annelida.net/ http://www.niwascience.co.nz/ncabb/ _______________________________________________ Annelida mailing list Post: Annelida@net.bio.net Help/archive: http://www.bio.net/biomail/listinfo/annelida Resources: http://www.annelida.net