[Annelida] Dried material
(by s.chambers from nms.ac.uk)
Wed Feb 25 06:37:29 EST 2009
I have tried the following method very successfully on a wide range of
invertebrates (Van Cleave H J and Ross J A 1947 A method for reclaiming
dried zoological specimens. Science 105(2725):318).
As mentioned in the method described by Daniel Martin you need to
monitor the re-hydration process as it depends on the permeability of
the cell membrane. As we all know different species respond differently
to a range of techniques.
RECONSTITUTION OF DRIED MATERIAL
1 Make up a solution of 0.25-0.5% Tri-sodium Orthophosphate in distilled
2 Place dried specimen in this solution and leave covered at room
temperature. Higher temperatures give a rapid reaction eg at 35 degrees
dried leeches were completely re-constituted in 2 days.
3 Occasionally examine specimen to observe when it has become
re-hydrated. Generally, polychaetes return to "normal" after 6-12 hours.
4 When specimen has returned to original size place in 30% alcohol for
approx 12 hours, then 50% alcohol, finally 74% alcohol.
5 You may find several air bubbles are trapped under the body wall
during the process and the specimen floats to the surface. These should
be removed and the specimen re-immersed in the tri-sodium Orthophosphate
It seems to work like magic!
Principal Curator of Marine Invertebrates
National Museums of Scotland
Edinburgh EH1 1JF
Tel: +44(0)131 247 4247
Fax:+44(0) 131 220 4819
Email: s.chambers from nms.ac.uk
From: annelida-bounces from oat.bio.indiana.edu
[mailto:annelida-bounces from oat.bio.indiana.edu] On Behalf Of Elena
Sent: 25 February 2009 05:06
To: Annelida List
Subject: [Annelida] Dried material
Dear friends and colleagues,
I wonder if you can suggest any chemicals to rehydrate dried polychaete
material. And if you had any experience with rehydrating, I would love
to hear about it.
Dr Elena Kupriyanova
Faculty of Education and Human Sciences
Yokohama National University
Tokiwadai, Hodogaya, Yokohama 240-8501, Japan
Tel and Fax +81 45 339 3408
e-mail: lena.kupriyanova from gmail.com
elenak from ynu.ac.jp
Annelida mailing list
Post: Annelida from net.bio.net
The world's greatest dressmaker. Jean Muir: A Fashion Icon.
National Museums Scotland, Scottish Charity, No. SC 011130
This communication is intended for the addressee(s) only. If you are not the addressee please inform the sender and delete the email from your system. The statements and opinions expressed in this message are those of the author and do not necessarily reflect those of National Museums Scotland. This message is subject to the Data Protection Act 1998 and Freedom of Information (Scotland) Act 2002. No liability is accepted for any harm that may be caused to your systems or data by this message.
More information about the Annelida