[Annelida] Dried material

[Susan Chambers]

Dear Elena 
I have tried the following method very successfully on a wide range of
invertebrates (Van Cleave H J and Ross J A 1947 A method for reclaiming
dried zoological specimens. Science 105(2725):318). 
As mentioned in the method described by Daniel Martin you need to
monitor the re-hydration process as it depends on the permeability of
the cell membrane. As we all know different species respond differently
to a range of techniques. 

RECONSTITUTION OF DRIED MATERIAL

1 Make up a solution of 0.25-0.5% Tri-sodium Orthophosphate in distilled
water. 
2 Place dried specimen in this solution and leave covered at room
temperature. Higher temperatures give a rapid reaction eg at 35 degrees
dried leeches were completely re-constituted in 2 days. 
3 Occasionally examine specimen to observe when it has become
re-hydrated. Generally, polychaetes return to "normal" after 6-12 hours.

4 When specimen has returned to original size  place in 30% alcohol for
approx 12 hours, then 50% alcohol, finally 74% alcohol. 
5 You may find several air bubbles are trapped under the body wall
during the process and the specimen floats to the surface. These should
be removed and the specimen re-immersed in the tri-sodium Orthophosphate
solution. 

It seems to work like magic!


Susan Chambers 
Principal Curator of Marine Invertebrates
National Museums of Scotland
Chambers Street
Edinburgh EH1 1JF
Scotland, UK
 
Tel: +44(0)131 247 4247
Fax:+44(0) 131 220 4819
Email: s.chambers from nms.ac.uk
Web:http://www.nms.ac.uk

-----Original Message-----
From: annelida-bounces from oat.bio.indiana.edu
[mailto:annelida-bounces from oat.bio.indiana.edu] On Behalf Of Elena
Kupriyanova
Sent: 25 February 2009 05:06
To: Annelida List
Subject: [Annelida] Dried material

Dear friends and colleagues,

I wonder if you can suggest any chemicals to rehydrate dried polychaete 
material. And if you had any experience with rehydrating, I would love 
to hear about it.
Thanks!
Lena

-- 
Dr Elena Kupriyanova
Faculty of Education and Human Sciences
Yokohama National University
Tokiwadai, Hodogaya, Yokohama 240-8501, Japan
Tel and Fax +81 45 339 3408
e-mail: lena.kupriyanova from gmail.com
elenak from ynu.ac.jp


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