[Annelida] Dried material

Joao Gil via annelida%40net.bio.net (by gil from ceab.csic.es)
Wed Feb 25 13:12:02 EST 2009


Hi,

While digging in my bibliography looking for another paper that insists to
remain missing, accidentally I have found this one. Maybe it is the solution
for your problems:

Jeppesen, Poul Christian. 1988. Use of vacuum in rehydratation of biological
tissue, with a review of liquids used. Crustaceana, 55 (3): 268-273. 

Here are the conclusions:

"The investigated biological material (mainly various crustaceans and
nereidid polychaetes) was most satisfactorily rehydrated by placing it in a
solution of Decon 90 or dioctyl sodium sulfosuccinate, subjecting it to the
repeated application of vacuum/equalization at room temperature for 24
hours, rinsing it in water and finally transferring it to the desired
preservative."

It must hurt...

João

João Gil
CEAB-CSIC
Carrer d'accés a la Cala Sant Francesc, 14
E-17300 BLANES (GIRONA)
SPAIN
Email: gil from ceab.csic.es
Telef. (34) 972.33.61.01
Fax: (34) 972.33.78.06

-----Mensaje original-----
De: annelida-bounces from oat.bio.indiana.edu
[mailto:annelida-bounces from oat.bio.indiana.edu] En nombre de Elena Kupriyanova
Enviado el: miércoles, 25 de febrero de 2009 6:06
Para: Annelida List
Asunto: [Annelida] Dried material

Dear friends and colleagues,

I wonder if you can suggest any chemicals to rehydrate dried polychaete 
material. And if you had any experience with rehydrating, I would love 
to hear about it.
Thanks!
Lena

-- 
Dr Elena Kupriyanova
Faculty of Education and Human Sciences
Yokohama National University
Tokiwadai, Hodogaya, Yokohama 240-8501, Japan
Tel and Fax +81 45 339 3408
e-mail: lena.kupriyanova from gmail.com
elenak from ynu.ac.jp


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