[Annelida] School in Molecular Zoology, White Sea Biological Station

Alexander Tzetlin via annelida%40net.bio.net (by atzetlin from gmail.com)
Wed Jan 14 08:41:19 EST 2009


Dear colleagues!
Please find the first announcement of the school of molecular zoology
at the White Sea Biological Station  of Lomonosov Moscow State
University.
Please help us with circulation of this announcement.

Alexander Tzetlin

Nikolay Pertsov White Sea Biological Station,

Faculty of Biology

Lomonosov Moscow State University

Vorobievy Gory, 1, build.12, Moscow 119991, Russia

Tel./fax in Moscow: +7 495 939 4495

Tel./fax at the White Sea Station: +7 815 64 516

Mobile phone: +7 911 317 3895

E-mail atzetlin from gmail.com

www.wsbs-msu.ru



"THE WHITE SEA MOLECULAR ZOOLOGY SUMMER SCHOOL"
at N.A. Pertzov White Sea Biological Station, Moscow State University
September, 6-20 2009

Announcement

N.A.Pertzov  White Sea Biological Station (WSBS MSU) invites
undergraduate and graduate students studying  zoology and ecology to
participate in summer field school on Molecular Zoology, which will
take place in September, 6-20 2009.

"THE WHITE SEA MOLECULAR ZOOLOGY SCHOOL" will provide a unique
opportunity to get on-hand experience of wide array of molecular
genetics methods and tools used in modern zoology. You are invited to
spend two weeks in marvelous natural settings and friendly environment
at small biological station located in pristine location called
"Velikaya Salma" ("Great straight") of the Kandalaksha Bay of the
White Sea exactly on the Polar Circle. Addition information about the
station can be found here:
http://wsbs-msu.ru/res/NEWS24/wsbs-presentation.pdf.

During two weeks you will be involved in organismal and ecological
fieldwork, laboratory data collection, and analytical work. The course
will emphasise the use of molecular tools to address ecological and
environmental questions.

The number of participants is limited to 12 people. Working language is
English

Limited financial support to cover accommodation and tuition will be
provided by the Biological Department of M.V. Lomonosov Moscow State
University and Russian Foundation of Basic Research, however travel
and meal expenses will be provided by the participants.
       The meal expenses will be near 10 euro per day. The price of train
ticket from Moscow to WSBS and back will be about 250 euro.

Syllabus


•       Faunistic survey of diverse marine fauna in different biotopes:
littoral fauna, benthic  study with grabs and corers,  plankton
sampling from research vessel;

•       Washing and processing and preliminary identification of benthic
samples, introduction to quantitative ecology sampling methods.

•       Overview of major groups of invertebrates inhabiting the White Sea,
determination of species with taxonomic keys, primer on zoological
drawings.

•       DNA extraction. Overview of applicability of different protocols.

•       PCR with standard barcoding (COI)  primers , agarose
electrophoresis, pre-sequencing PCR-product purification

•       PCR-RFLP analysis for species identification

•       PCR with microsatellite (SSR) primers, - acrylamide electrophoresis,
EtBr staining.

•       Demonstration of other methods such as TA-cloning of PCR products,
RNA extraction and RT-PCR (optional, subject to availability of time,
reagents, equipment and luck supply)

•       Sequence analysis. Contig construction, quality checking of
nucleotide sequence, and BLAST and BLAT algorithms.

•       Molecular Genetic databanks (GenBank etc.,) and other available
resources on the Internet.

•       Discussion Barcoding approaches and approaches of sequence
identification.

•       Introduction to molecular ecology and population genetics analyses
including estimation of allele frequency of microsatellite loci,
statistical analysis of population differentiation, coalescence
approaches.

•       Introduction to phylogenetic analysis. (PAUP, Mr.Bayes, PAML, etc.)

Morning hours lectures (9:30 am – noon will be followed by laboratory
practicals (1 pm till 7 pm), except the days dedicated to full-day or
half-day excursions for specimens collection, either on board of
research vessel "Belomor" or from the shore on littoral zone. Exact
schedule of field trips will be adjusted according to weather and
tides.



Tentative list of lecturers

Dr. Ken Halianych  http://gump.auburn.edu/halanych/lab/
Dr. Mikhail Matz  http://www.biosci.utexas.edu/ib/faculty/matz.htm
Dr. Alex Kondrashov
http://www.eeb.lsa.umich.edu/eeb/people/kondrash/index.html
Dr.Tatiana Azhikina (Institute of Bioorganic Chemistry, Moscow)
http://humgen.siobc.ras.ru/people/people.html
Dr.Tatiana Neretina (WSBS) http://wsbs-msu.ru/doc/index.php?ID=22
Dr.Anna Zhadan (WSBS) http://wsbs-msu.ru/doc/view.php?ID=44
Prof. Yuriy Lebedev (Institute of Bioorganic Chemistry, Moscow)
http://labcfg.ibch.ru/index.html
Dr. Nikolai Mugue (Institute of Developmental Biology RAS / VNIRO,
Moscow) http://idbras.comcor.ru/indexe.HTM

Contact e-mail: wsbs.school from gmail.com
Contact person: Tatyana Neretina
For participation in this school please fill the form and send it
before the 30th of March, 2009.


PRE-REGISTRATION FORM

Name    ………………………………………………………………………..

Affiliation...........................................................................................................

Taken classes ………………………………………………………………….

Experience of fieldwork …………………………………………………………

Why are you interesting in this summer school?
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