From Lizbeth_Kelly from hgsi.com Mon Jul 6 10:41:06 2009 From: Lizbeth_Kelly from hgsi.com (Lizbeth_Kelly@hgsi.com) Date: Mon Jul 6 14:49:38 2009 Subject: [Annelida] Sorensen's Buffer recipe Message-ID: Annelida, Could you share the recipe for Sorensen's Buffer? Thanks, Lizbeth Kelly, HT(ASCP), Q-IHC Histology Department Human Genome Sciences, Inc. 14200 Shady Grove Road Rockville, MD 20850 240-314-4400 X2325 Fax: 301-354-4176 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/annelida/attachments/20090706/ab632825/attachment.html From g.read from niwa.co.nz Mon Jul 6 15:35:42 2009 From: g.read from niwa.co.nz (Geoff Read) Date: Mon Jul 6 15:38:27 2009 Subject: [Annelida] Sorensen's Buffer recipe In-Reply-To: References: Message-ID: <4A5308DD.8045.00D5.0@niwa.co.nz> Hi Lizbeth, This was asked some years ago and the reply then from Tom Parker was: "A recipe for the phosphate buffer is in Humanson's, "Animal Tissue Techniques" Fourth edition, page 556." To which I can add (in these more digitalised times) http://books.google.com/books?id=5OdqAAAAMAAJ&source=gbs_ViewAPI Sounds like a good read. There's a 1997 5th edition, and prior editions will still be in copyright. I'm sure it's widely available in academic libraries and second hand. We (niwa) seem to have the 3rd edition. I can check for it if you're really stuck and can't get it elsewhere. Geoff >>> On 7/07/2009 at 3:41 a.m., wrote: > Annelida, > > Could you share the recipe for Sorensen's Buffer? > Thanks, > > Lizbeth Kelly, HT(ASCP), Q-IHC > Histology Department > Human Genome Sciences, Inc. > 14200 Shady Grove Road > Rockville, MD 20850 > 240-314-4400 X2325 > Fax: 301-354-4176 -- Geoff Read http://www.annelida.net/ http://www.niwa.co.nz/about-niwa *************************** NIWA is the trading name of the National Institute of Water & Atmospheric Research Ltd. From schulzea from tamug.edu Mon Jul 6 16:20:48 2009 From: schulzea from tamug.edu (Anja Schulze) Date: Mon Jul 6 16:24:55 2009 Subject: [Annelida] Sorensen's Buffer recipe Message-ID: <7D83578C31ACF643AC8639465A2A720A016800@tamugex.tamug.edu> Hi Lizbeth, In case you cannot find the reference, this is the protocol that I use. It will make 500 ml of 0.2 M buffer, pH 7.4. Very simple. Reagents: Sodium phosphate monobasic (NaH2PO4 * H2O) Sodium phosphate dibasic (Na2HPO4) Mix 100 ml 0.1M sodium phosphate monobasic with 400 ml of 0.1M sodium phosphate dibasic. Store at 4? C. Reference: Clark, G., W. J. Dougherty, and F. H. Kasten. 1981. General Methods, pp 1-103 in Clark, G. (ed.) Staining procedures. Williams & Wilkins, Baltimore. Good luck! Anja Schulze, Ph.D Assistant Professor Department of Marine Biology Texas A&M University at Galveston 5007 Avenue U Galveston, TX 77551 Phone: 409-740-4540 Email: schulzea@tamug.edu http://www.tamug.edu/schulze/ -----Original Message----- From: annelida-bounces@oat.bio.indiana.edu [mailto:annelida-bounces@oat.bio.indiana.edu] On Behalf Of Geoff Read Sent: Monday, July 06, 2009 3:36 PM To: Lizbeth_Kelly@hgsi.com; annelida@magpie.bio.indiana.edu Subject: Re: [Annelida] Sorensen's Buffer recipe Hi Lizbeth, This was asked some years ago and the reply then from Tom Parker was: "A recipe for the phosphate buffer is in Humanson's, "Animal Tissue Techniques" Fourth edition, page 556." To which I can add (in these more digitalised times) http://books.google.com/books?id=5OdqAAAAMAAJ&source=gbs_ViewAPI Sounds like a good read. There's a 1997 5th edition, and prior editions will still be in copyright. I'm sure it's widely available in academic libraries and second hand. We (niwa) seem to have the 3rd edition. I can check for it if you're really stuck and can't get it elsewhere. Geoff >>> On 7/07/2009 at 3:41 a.m., wrote: > Annelida, > > Could you share the recipe for Sorensen's Buffer? > Thanks, > > Lizbeth Kelly, HT(ASCP), Q-IHC > Histology Department > Human Genome Sciences, Inc. > 14200 Shady Grove Road > Rockville, MD 20850 > 240-314-4400 X2325 > Fax: 301-354-4176 -- Geoff Read http://www.annelida.net/ http://www.niwa.co.nz/about-niwa *************************** NIWA is the trading name of the National Institute of Water & Atmospheric Research Ltd. _______________________________________________ Annelida mailing list Post: Annelida@net.bio.net Help/archive: http://www.bio.net/biomail/listinfo/annelida Resources: http://www.annelida.net From g.read from niwa.co.nz Thu Jul 9 23:59:53 2009 From: g.read from niwa.co.nz (Geoff Read) Date: Fri Jul 10 00:03:38 2009 Subject: [Annelida] Goloboff et al on annelids? Message-ID: <4A577388.8045.00D5.0@niwa.co.nz> Hi all, Has anyone looked at the data of Goloboff et al. 2009 for the "worm" placements (mentioned on taxacom today)? Goloboff PA, Catalano SA, Mirande JM, Szumik CA, Arias JS, K?llersj? M, Farris JS 2009. Phylogenetic analysis of 73 060 taxa corroborates major eukaryotic groups. Cladistics 25: 211-230. The analysis took ~ 2.5 months processor-time and included 942 annelids. Annelida and Mollusca are not shown in their figure (at the scale given). But they say their tree has "Scaphopoda and Bivalvia as successive sister groups of several other invertebrate phyla, and the rest of the molluscan classes forming a monophyletic group." "The full data set and the trees found (in TNT format), as well as the C scripts used to download GenBank sequences, can be obtained from http://www.zmuc.dk/ public/phylogeny/TNT/More/Supp_Data_Set.tgz" Is anyone knowing TNT keen to have a look & report? Geoff -- Geoff Read http://www.annelida.net/ http://www.niwa.co.nz/about-niwa *************************** NIWA is the trading name of the National Institute of Water & Atmospheric Research Ltd. From myhk2 from cam.ac.uk Mon Jul 13 04:19:50 2009 From: myhk2 from cam.ac.uk (Matthew Kuo) Date: Mon Jul 13 05:11:09 2009 Subject: [Annelida] Deep sea worms...shallow marine worms In-Reply-To: <4A577388.8045.00D5.0@niwa.co.nz> References: <4A577388.8045.00D5.0@niwa.co.nz> Message-ID: <4A5AFC36.4010102@cam.ac.uk> Dear All, A couple of unusual questions: is anyone familiar with burrowing invertebrate species that pelletize (fecal pellets of 3-5mm diameter) deep sea soft clay sediments; and if there are species that produce similar-sized pellets in shallow marine/tidal (mud flats) environments? Can these shallow marine species be readily 'obtained' and 'farmed' in the laboratory? Many thanks for your help. Matthew. ----------------------------------------------------------- Matthew Kuo Research Student Geotechnical and Environmental Engineering Group Cambridge University Engineering Department Phone +44 (0) 1223 766686 Mobile +44 (0) 7902 788835 Website http://www2.eng.cam.ac.uk/~myhk2/ ----------------------------------------------------------- From g.read from niwa.co.nz Wed Jul 15 20:16:53 2009 From: g.read from niwa.co.nz (Geoff Read) Date: Wed Jul 15 20:19:09 2009 Subject: [Annelida] Palouse earthworm hunt Message-ID: <4A5F2845.8045.00D5.0@niwa.co.nz> FYI Press story. Searchers shovel northwest dirt seeking giant worm http://news.yahoo.com/s/ap/20090711/ap_on_sc/us_giant_palouse_earthworm Documented collections of the species, known locally as GPE, have occurred only in 1978, 1988, 1990 and 2005. http://www.palouseprairie.org/invertebrates/palouseworm.html -- Geoff Read http://www.annelida.net/ http://www.niwa.co.nz/about-niwa *************************** NIWA is the trading name of the National Institute of Water & Atmospheric Research Ltd.