[Annelida] Sorensen's Buffer recipe
(by schulzea from tamug.edu)
Mon Jul 6 16:20:48 EST 2009
In case you cannot find the reference, this is the protocol that I use. It will make 500 ml of 0.2 M buffer, pH 7.4. Very simple.
Sodium phosphate monobasic (NaH2PO4 * H2O)
Sodium phosphate dibasic (Na2HPO4)
Mix 100 ml 0.1M sodium phosphate monobasic with 400 ml of 0.1M sodium phosphate dibasic. Store at 4º C.
Reference: Clark, G., W. J. Dougherty, and F. H. Kasten. 1981. General Methods, pp 1-103 in Clark, G. (ed.) Staining procedures. Williams & Wilkins, Baltimore.
Anja Schulze, Ph.D
Department of Marine Biology
Texas A&M University at Galveston
5007 Avenue U
Galveston, TX 77551
Email: schulzea from tamug.edu
From: annelida-bounces from oat.bio.indiana.edu [mailto:annelida-bounces from oat.bio.indiana.edu] On Behalf Of Geoff Read
Sent: Monday, July 06, 2009 3:36 PM
To: Lizbeth_Kelly from hgsi.com; annelida from magpie.bio.indiana.edu
Subject: Re: [Annelida] Sorensen's Buffer recipe
This was asked some years ago and the reply then from Tom Parker was:
"A recipe for the phosphate buffer is in Humanson's, "Animal Tissue
Techniques" Fourth edition, page 556."
To which I can add (in these more digitalised times)
Sounds like a good read. There's a 1997 5th edition, and prior editions will still be in copyright. I'm sure it's widely available in academic libraries and second hand.
We (niwa) seem to have the 3rd edition. I can check for it if you're really stuck and can't get it elsewhere.
>>> On 7/07/2009 at 3:41 a.m., <Lizbeth_Kelly from hgsi.com> wrote:
> Could you share the recipe for Sorensen's Buffer?
> Lizbeth Kelly, HT(ASCP), Q-IHC
> Histology Department
> Human Genome Sciences, Inc.
> 14200 Shady Grove Road
> Rockville, MD 20850
> 240-314-4400 X2325
> Fax: 301-354-4176
Geoff Read <g.read from niwa.co.nz>
NIWA is the trading name of the National Institute of Water & Atmospheric Research Ltd.
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