[Annelida] Sorensen's Buffer recipe

[Anja Schulze]

Hi Lizbeth,

In case you cannot find the reference, this is the protocol that I use. It will make 500 ml of 0.2 M buffer, pH 7.4. Very simple.

Reagents:
Sodium phosphate monobasic (NaH2PO4 * H2O)
Sodium phosphate dibasic (Na2HPO4)

Mix 100 ml 0.1M sodium phosphate monobasic with 400 ml of 0.1M sodium phosphate dibasic. Store at 4º C.

Reference: Clark, G., W. J. Dougherty, and F. H. Kasten. 1981. General Methods, pp 1-103 in Clark, G. (ed.) Staining procedures. Williams & Wilkins, Baltimore.

Good luck!

Anja Schulze, Ph.D
Assistant Professor
Department of Marine Biology
Texas A&M University at Galveston
5007 Avenue U
Galveston, TX 77551
Phone: 409-740-4540
Email: schulzea from tamug.edu
http://www.tamug.edu/schulze/
 

-----Original Message-----
From: annelida-bounces from oat.bio.indiana.edu [mailto:annelida-bounces from oat.bio.indiana.edu] On Behalf Of Geoff Read
Sent: Monday, July 06, 2009 3:36 PM
To: Lizbeth_Kelly from hgsi.com; annelida from magpie.bio.indiana.edu
Subject: Re: [Annelida] Sorensen's Buffer recipe

Hi Lizbeth,

This was asked some years ago and the reply then from Tom Parker was:

"A recipe for the phosphate buffer is in Humanson's, "Animal Tissue
Techniques" Fourth edition, page 556."

To which I can add (in these more digitalised times)

http://books.google.com/books?id=5OdqAAAAMAAJ&source=gbs_ViewAPI

Sounds like a good read. There's a 1997 5th edition, and prior editions will still be in copyright. I'm sure it's widely available in academic libraries and second hand.

We (niwa) seem to have the 3rd edition. I can check for it if you're really stuck and can't get it elsewhere. 

Geoff


>>> On 7/07/2009 at 3:41 a.m., <Lizbeth_Kelly from hgsi.com> wrote:
> Annelida,
> 
> Could you share the recipe for Sorensen's Buffer?
> Thanks,
> 
> Lizbeth Kelly, HT(ASCP), Q-IHC
> Histology Department
> Human Genome Sciences, Inc.
> 14200 Shady Grove Road
> Rockville, MD  20850
> 240-314-4400 X2325
> Fax: 301-354-4176


-- 

 Geoff Read <g.read from niwa.co.nz>
  http://www.annelida.net/
  http://www.niwa.co.nz/about-niwa
 ***************************


NIWA is the trading name of the National Institute of Water & Atmospheric Research Ltd.

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