[Annelida] Mitochondrial isolation for mt DNA extraction from Earthworms

Luís Cunha via annelida%40net.bio.net (by luisnevescunha from gmail.com)
Sun Oct 4 23:30:39 EST 2009


Dear collegues
I wonder if anyone ever tried to isolate mitochondrias from earthworms for
posterior mtDNA extraction, could anyone sen me a good protocol? I am having
a lot of trouble doing that, probably due to soil contamination in the
digestive tract, even after depuration and I am getting very low yield of
mtDNA. Any help?? I am working with Pontoscolex corethrurus, my model
organism :).

Best regards

-- 
Luís Cunha
(Msc, Phd student)
Centro de Investigação em Recursos Naturais, Departamento de Biologia –
Universidade dos Açores, Rua da Mãe de Deus, 13A – Apartado 1422, 9501-855
Ponta Delgada
Açores, Portugal
Tel: (+351) 296 650 102 (ext. 1476)
Fax: (+351) 296 650 100
Tlm (+351) 913897557
E-mail: luisnevescunha from gmail.com
http://www.pherg.org
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