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[Annelida] SEM of larvae

Dieter Fiege via annelida%40net.bio.net (by Dieter.Fiege from senckenberg.de)
Thu Apr 1 10:09:42 EST 2010

Dear colleagues,

does anyone have experience/a protocoll for SEMing planktonic larvae
that have been fixed in Steedman's fixative and then transferred to
Steedman's preservative? Into which percentage of ethanol do I transfer
them as a start for the graded ethanol series folowed by critical point
drying without doing any harm (shrinkage) to them. I have checked some
literature and googled but couldn't find anything.

All hints welcome!

Happy Easter!


Steedman’s Preservative,  Johnson and Allen (2005)
1.  95% water, RO or tap water ok
2.  4.5% 1,2 propanediol (propylene glycol)
3.  0.5% propylene phenoxytol

You can make a concentrate as follows:
90 mL propylene glycol
10 mL propylene phenoxytol

Add 5% of the concentrate to 95% water.

Steedman’s Fixative
1.  50% Formalin (40% formaldehyde) buffered with borax (20-30 g to 1
liter of full strength formalin)
2.  45% 1,2 propanediol
3.  5% propylene phenoxytol

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