[Annelida] Problems with sequencing CO1 in polychaetes

maite.aguado from uam.es via annelida%40net.bio.net (by maite.aguado from uam.es)
Tue Jun 7 03:01:38 EST 2011


Dear Elena,
Sequencing COI in syllids has been extremely difficult. I got some  
results but very few in comparison with the number of times I tried  
modifying almost everything in the protocols. I designed new primers,  
which was also difficult, and they were not more succesful. We'll  
continue trying anyway.
Good luck!
Cheers,
Maite Aguado

Elena Kupriyanova <Elena.Kupriyanova from austmus.gov.au> escribió:

> Dear friends and colleagues,
>
>
>
> I accidentally sent the question below from my old (non-functional)  
> email.  Could those of you who kindly replied, repeat it to this my  
> account please. Just to clarify - I need examples of groups where  
> sequencing CO1 using "universal" Folmer primers was impossible and  
> designing new primers was difficult. The is the case with serpulid  
> and I want to know how common the situation is in polychaetes.
>
> Thank you!
>
> Lena
>
> **********************************
>
> Dear all,
>
>
>
> Please let me know if you had difficulties sequencing the barcoding  
> fragment of CO1 in your favourite polychaete groups. Have those  
> problems been resolved? Has this info been published? Any info is  
> highly appreciated.
>
>
>
> Wormly,
>
> Lena
>
>
>
>
> Dr. Elena Kupriyanova
> Marine Invertebrates
> Australian Museum
> 6 College Street Sydney NSW 2010 Australia
> t 61 2 9320 6340   m 61402735679   f 61 2 9320 6059
> www.australianmuseum.net.au
>
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