Dear Elena,
Sequencing COI in syllids has been extremely difficult. I got some
results but very few in comparison with the number of times I tried
modifying almost everything in the protocols. I designed new primers,
which was also difficult, and they were not more succesful. We'll
continue trying anyway.
Good luck!
Cheers,
Maite Aguado
Elena Kupriyanova <Elena.Kupriyanova from austmus.gov.au> escribió:
> Dear friends and colleagues,
>>>> I accidentally sent the question below from my old (non-functional)
> email. Could those of you who kindly replied, repeat it to this my
> account please. Just to clarify - I need examples of groups where
> sequencing CO1 using "universal" Folmer primers was impossible and
> designing new primers was difficult. The is the case with serpulid
> and I want to know how common the situation is in polychaetes.
>> Thank you!
>> Lena
>> **********************************
>> Dear all,
>>>> Please let me know if you had difficulties sequencing the barcoding
> fragment of CO1 in your favourite polychaete groups. Have those
> problems been resolved? Has this info been published? Any info is
> highly appreciated.
>>>> Wormly,
>> Lena
>>>>> Dr. Elena Kupriyanova
> Marine Invertebrates
> Australian Museum
> 6 College Street Sydney NSW 2010 Australia
> t 61 2 9320 6340 m 61402735679 f 61 2 9320 6059
> www.australianmuseum.net.au
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