[Annelida] WormNet II - Community Sequencing Project

Anja Schulze via annelida%40net.bio.net (by schulzea from tamug.edu)
Mon Mar 7 18:28:28 EST 2011


WormNet II - Community Sequencing Project

Dear Colleagues,

We are pleased to announce a community-based sequencing service for annelids within the framework of the recently awarded NSF AToL Grant "WormNet II - Assembling the Annelid Tree of Life". The project is intended to resolve phylogenetic relationships among closely related annelid species and to investigate genetic diversity within selected species and complexes of cryptic species. The service is available at no charge to researchers who can supply appropriately preserved material.

What we offer:
*             Sequencing of the two mitochondrial markers cytochrome c oxidase subunit I (COI) and 16S ribosomal RNA for approximately 3000 annelid samples, supplied by the community over a five-year period. Samples may consist of whole specimens, tissue samples or cleaned PCR products. Species not represented in GenBank will have priority.
*             Databasing of collection information and sequence data in a centralized databank at Auburn University
*             Collaboration on data analysis and publication
*             Deposition of voucher material in museum collections, if applicable

Interested parties are requested to supply a short description of the intended project, including the approximate number and type of samples, preservation method, permit requirements and status, shipping needs and logistics, plans for deposition of voucher material in museum collections (if not done by processing lab) and expected time frame for processing, data analysis and publication. A sample proposal is pasted below. Details of authorship of any resulting publications will be negotiated on a case-by-case basis. A Memorandum of Understanding between the party supplying the samples and the processing lab will outline the responsibilities of each party.

Interested researchers should contact:

Anja Schulze, Texas A&M University at Galveston; Email: schulzea from tamug.edu
Or:
Frank Anderson, Southern Illinois University; Email: feander from siu.edu

Sample Proposal:
My Ph.D. student is currently working on a morphological data set for the eunicid polychaete genus Palola. As the species in this genus are morphologically very similar we need to add molecular data to analyze the phylogeny of the genus. We have a total 40 specimens fixed and preserved in 95% ethanol which we think represent five different species. The specimens were collected in Belize, Panama, Palau and the Federated States of Micronesia in 2008. Copies of the respective collection and export permits will be provided. We will send whole specimens by regular mail in small amounts of ethanol. We have not made arrangements for deposition of voucher material in museum collections and would appreciate if you could assist us in this matter. We request the COI and 16S sequence data by Sept. 2011 because the student has to submit a manuscript draft by Dec. 2011. We may also require assistance with data analysis.

Anja Schulze, Ph.D.
Assistant Professor, Marine Biology
Texas A&M University at Galveston
200 Seawolf Parkway
OCSB Bldg 3029, Rm 258
Galveston, TX 77553
Phone: 409-740-4540
Fax: 409-740-5001
schulzea from tamug.edu

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