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[Annelida] substitution for formalin

Geoff Read via annelida%40net.bio.net (by Geoffrey.Read from niwa.co.nz)
Fri Feb 24 20:36:08 EST 2012

My view is that, like a visit to the dentist, formalin fixation is an unpleasant but necessary process for long term protection from decay.  Ethanol as a fixative at proper concentration renders specimens so rigid and brittle they are almost unworkable with - except for DNA extraction, and at lower concentrations you can get unidentifiable mush.  Proprietory fixative formulas of unknown recipe are around. Trust a secret recipe - maybe not - and price might be a limitation.


From: annelida-bounces from oat.bio.indiana.edu [annelida-bounces from oat.bio.indiana.edu] on behalf of Doug Strom [dstrom from waterandair.com]
Sent: 25 February 2012 10:15
To: Polychaete mailing list
Subject: Re: [Annelida] substitution for formalin

The USEPA has started using a product called NOTOXHisto:


For the samples we have seen it appears to fix macroinvertebrates and in particular polychaetes pretty well.


[I sent this reply to Barbara, but meant it for the group as well. - DGS]

Doug Strom
Project Scientist - Aquatic Ecologist
Water & Air Research Inc.
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dstrom from waterandair.com<http://www.waterandair.com/>
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On Fri, Feb 24, 2012 at 5:50 AM, Barbara Mikac <barbara.mikac from cim.irb.hr<mailto:barbara.mikac from cim.irb.hr>> wrote:
Dear colleagues,

I am interested if there is some other fixative for the macrozoobenthic
samples that can substitute formalin? All groups will be analysed
analyses later but special interest will be in polychaetes.

Thank you very much for your help!


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