Our company looked at an alternative fixative called NOTOX Histo.
We found that it was fine as a fixative for macroinvertebrates up to the
size of edible shrimp. We did find that there may be matrix interference,
so if there was a lot of organic matter like leaves in the sample it did
not perform as well.
Conversely, we found it worked best on specimens preserved separately from
the sample matrix.
*I attached a presentation and poster about our evaluation efforts. I hope
you got them at your e-mail address, since that message to Annelida_L
failed. Any others interested in those materials please contact me
directly.*
The Florida Department of Environmental Protection reviewed our data,
performed their own study, and found that it was an acceptable alternative
t formalin. It has been added t the State QA rule.
So I recommend looking into that preservative.
We have not evaluated whether this substance will allow genetic DNA
analyses, but we hope to pursue that question in the future. We did
preserve some vouchers in it for a study including genetic analyses being
conducted by the University of Florida
Cheers,
Doug
------------------------------
Doug Strom
Project Scientist - Aquatic Ecologist
Water & Air Research Inc.
6821 SW Archer Road
Gainesville, Florida 32608
Toll Free: 800.242.4927
Direct: 352.224.1555
Local: 352.372.1500 Extension 186
Cell: 352.538.1063
Fax: 352.378.1500
dstrom from waterandair.com <http://www.waterandair.com/>
www.waterandair.com
On Wed, Feb 14, 2018 at 5:19 PM, Doug Strom <dstrom from waterandair.com> wrote:
> Our company looked at an alternative fixative called NOTOX Histo.
>> We found that it was fine as a fixative for macroinvertebrates up to the
> size of edible shrimp. We did find that there may be matrix interference,
> so if there was a lot of organic matter like leaves in the sample it did
> not perform as well.
>> Conversely, we found it worked best on specimens preserved separately from
> the sample matrix.
>> See attached a presentation and poster about our evaluation efforts.
>> The Florida Department of Environmental Protection reviewed our data,
> performed their own study, and found that it was an acceptable alternative
> t formalin. It has been added t the State QA rule.
>> So I recommend looking into that preservative.
>> We have not evaluated whether this substance will allow genetic DNA
> analyses, but we hope to pursue that question in the future. We did
> preserve some vouchers in it for a study including genetic analyses being
> conducted by the University of Florida
>> Cheers,
>> Doug
> ___________________________________
>>> ------------------------------
> Doug Strom
> Project Scientist - Aquatic Ecologist
> Water & Air Research Inc.
> 6821 SW Archer Road
> Gainesville, Florida 32608
> Toll Free: 800.242.4927 <(800)%20242-4927>
> Direct: 352.224.1555 <(352)%20224-1555>
> Local: 352.372.1500 Extension 186 <(352)%20372-1500>
> Cell: 352.538.1063 <(352)%20538-1063>
> Fax: 352.378.1500 <(352)%20378-1500>
>dstrom from waterandair.com <http://www.waterandair.com/>
> www.waterandair.com
>> On Wed, Feb 14, 2018 at 4:51 PM, Vivianne Solis <viviannesw from gmail.com>
> wrote:
>>> Dear Annelidans:
>> It came to my attention, that despite the traditional fixing in formalin,
>> when collecting polychaetes recommended by Fauchald all his life, in order
>> to preserve the organisms that otherwise become wasted in the medium term,
>> because alcohol does not penetrate their tissues enough, now that
>> substance
>> appears to be forbidden in Europe.
>>>> If so, what are you using to fix them before storing them in alcohol?
>>>> Thanks for the feedback
>>>> Wormly
>>>> Vivianne
>>>> <https://www.avast.com/sig-email?utm_medium=email&utm_source>> =link&utm_campaign=sig-email&utm_content=webmail>
>> Libre
>> de virus. www.avast.com
>> <https://www.avast.com/sig-email?utm_medium=email&utm_source>> =link&utm_campaign=sig-email&utm_content=webmail>
>> <#DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9FDF2>
>> _______________________________________________
>> Annelida mailing list
>> Post: Annelida from net.bio.net>> Help/archive: http://www.bio.net/biomail/listinfo/annelida>> Resources: http://www.annelida.net>>>>
