Dear Annelideans!
Thank you very much for your advices!
I will try to follow them all:
*Streaming water, a bath of distilled water, drops of a contact lens
cleaner, ultrasonic cleaner and **solution of white vinegar and 70% ethano*
l.
Especial thanks to Katie for the useful paper.
If someone know something else – please write to me!
Sincerely,
Nataly
2018-02-15 15:57 GMT+03:00 Sergio Salazar <savs551216 from hotmail.com>:
> Dear Nataly,
>>> We use a 1:1 solution of white vinegar and 70% ethanol. Specimens are
> placed in this solution for a few minutes, and then they are carefully
> brushed with a small camel-hair brush. Most salt crystals and adsorbed
> organic matter will be removed. Chaetae will look very neat, indeed.
> However, if the specimen was not properly fixed, ciliated areas will be
> damaged as well. Likewise, if salt crystals have been adsorbed during many
> years, they will be very difficult to be removed.
>>> An alternative might be to reduce the proportion of vinegar, or to reduce
> the number of brushes over delicate areas/worms.
>>> Best wishes,
>>> Sergio
>>> ------------------------------
> *De:* annelida-bounces from oat.bio.indiana.edu <annelida-bounces from oat.bio.> indiana.edu> en nombre de Наталия Днестровская <ndnestro from gmail.com>
> *Enviado:* miércoles, 14 de febrero de 2018 01:08 p. m.
> *Para:* annelida from magpie.bio.indiana.edu> *Asunto:* [Annelida] How to clean the chaetae?
>> Dear Annelideans!
>> I have a problem and want to ask you for advice.
>>> The worms that I received from my colleague were covered by *very small*
> crysltals or parts of something.
>>> The body and chaetae all together – see the photos.
>> So I can’t consider the shape of spines
>>>> How dissolve them – to make good SEM photo ?
>>>> Sincerely
>> Nataly
>>>> --
> Nataliya Dnestrovskaya
> Senior Researcher
> Dept of Hydrobiology
> Moscow State University
>http://hydro.bio.msu.ru/>
--
Nataliya Dnestrovskaya
Senior Researcher
Dept of Hydrobiology
Moscow State University
http://hydro.bio.msu.ru/
