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[Annelida] anybody able to help - staining worms

Pat Hutchings via annelida%40net.bio.net (by Pat.Hutchings from austmus.gov.au)
Thu Jan 3 17:18:48 EST 2019


many thanks will forward it to him Pat


Dr Pat Hutchings
Senior Fellow Australian Museum Research Institute

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________________________________
From: annelida-bounces from oat.bio.indiana.edu <annelida-bounces from oat.bio.indiana.edu> on behalf of Eduardo Zattara <ezattara from gmail.com>
Sent: Thursday, 3 January 2019 11:44:55 PM
To: Annelida from magpie.bio.indiana.edu
Subject: Re: [Annelida] anybody able to help - staining worms

I have not tried for long term staining worms, but for larger species, the
fluorescent elastomers used in amphibians might work nicely. The are
injected under the skin with a syringe, and tend to stay in place and be
visible through the epidermis, so you can combine color dots to achieve
group or even individual labeling schemes.

Cheers!

     -Ed-

"Only in silence the word,
only in dark the light,
only in dying life :
bright the hawk's flight on the empty sky"

--------------
Dr. Eduardo E. Zattara

*Adjunct Researcher*
Instituto de Investigaciones en Biodiversidad y Medio Ambiente
Centro Regional Universitario Bariloche, Universidad Nacional del Comahue

*Consejo Nacional de Investigaciones Científicas y Tecnológicas*
*Research Collaborator*
Dept. of Invertebrate Zoology, National Museum of Natural History
*Smithsonian Institution*

*Research Associate*
Department of Biology, Indiana Molecular Biology Institute
*Indiana University, Bloomington*

email: ezattara from gmail.com <ezattara from umd.edu>
phone: +54 294 461 7958
mail address: Quintral 1250, Bariloche, Rio Negro 8400, Argentina
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On Thu, Jan 3, 2019 at 7:04 AM Paulo Lana <lana from ufpr.br> wrote:

> Hi Pat and colleagues,
>
> We have successfully tried Neutral Red  with nematodes. Please have a
> look at:
>
>
> https://apac01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.researchgate.net%2Fpublication%2F259228576_A_new_look_into_the_small-scale_dispersal_of_free-living_marine_nematodes&amp;data=02%7C01%7Cpat.hutchings%40austmus.gov.au%7Cbc9c95b49d284d2d429808d6717a611a%7C6ee75868f5d64c8cb4cda3ddce30cfd6%7C0%7C0%7C636821167775603262&amp;sdata=3lkccxEkQ9lODv17YkmO%2BtKZrXOcBhIw0ZcYIV0Yr0w%3D&amp;reserved=0
>
> Regards,
>
> Paulo Lana
>
> On 03/01/2019 04:21, Pat Hutchings wrote:
> > Hi folks
> >
> > a colleague of mine has asked me if I know any such stains
> >
> >
> > "My colleague has asked me the following question, as I am having very
> poor luck in a literature search for mark-recapture methods for marine
> worms. There is an emerging field of work on chemical tracers and stains
> for the mark-recapture technique, particularly for calcifiers like crabs
> and molluscs (e.g. using Calcein). Do you know of any people/papers that
> had success staining worms for recapture and/or successful alternative
> methods?"
> >
> >
> > I had to confess that I had no knowledge of such a stain
> >
> >
> > anyhow if anybody knows - of such stains please can they let me know
> thanks Pat
> >
> >
> >
> >
> > Dr Pat Hutchings
> > Senior Fellow Australian Museum Research Institute
> >
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