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[Annelida] anybody able to help - staining worms

Prof. PY Qian via annelida%40net.bio.net (by boqianpy from ust.hk)
Mon Jan 7 03:36:56 EST 2019


Just returned to my office from a trip.  I used neutral red for a study 
during my PhD thesis study and the work was published in MEPS in 1994.  
It worked well (without mortality).  PY

.  Pat Hutchings wrote:
> many thanks will forward it to him Pat
>
>
> Dr Pat Hutchings
> Senior Fellow Australian Museum Research Institute
>
> Visit: http://www.australianmuseum.net.au<https://webmail.austmus.gov.au/owa/redir.aspx?C=e7348c0a0d29466da8081316934e9a3b&URL=http%3a%2f%2fwww.australianmuseum.net.au%2f>
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> ________________________________
> From: annelida-bounces from oat.bio.indiana.edu <annelida-bounces from oat.bio.indiana.edu> on behalf of Eduardo Zattara <ezattara from gmail.com>
> Sent: Thursday, 3 January 2019 11:44:55 PM
> To: Annelida from magpie.bio.indiana.edu
> Subject: Re: [Annelida] anybody able to help - staining worms
>
> I have not tried for long term staining worms, but for larger species, the
> fluorescent elastomers used in amphibians might work nicely. The are
> injected under the skin with a syringe, and tend to stay in place and be
> visible through the epidermis, so you can combine color dots to achieve
> group or even individual labeling schemes.
>
> Cheers!
>
>       -Ed-
>
> "Only in silence the word,
> only in dark the light,
> only in dying life :
> bright the hawk's flight on the empty sky"
>
> --------------
> Dr. Eduardo E. Zattara
>
> *Adjunct Researcher*
> Instituto de Investigaciones en Biodiversidad y Medio Ambiente
> Centro Regional Universitario Bariloche, Universidad Nacional del Comahue
>
> *Consejo Nacional de Investigaciones Científicas y Tecnológicas*
> *Research Collaborator*
> Dept. of Invertebrate Zoology, National Museum of Natural History
> *Smithsonian Institution*
>
> *Research Associate*
> Department of Biology, Indiana Molecular Biology Institute
> *Indiana University, Bloomington*
>
> email: ezattara from gmail.com <ezattara from umd.edu>
> phone: +54 294 461 7958
> mail address: Quintral 1250, Bariloche, Rio Negro 8400, Argentina
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>
>
>
> On Thu, Jan 3, 2019 at 7:04 AM Paulo Lana <lana from ufpr.br> wrote:
>
>> Hi Pat and colleagues,
>>
>> We have successfully tried Neutral Red  with nematodes. Please have a
>> look at:
>>
>>
>> https://apac01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.researchgate.net%2Fpublication%2F259228576_A_new_look_into_the_small-scale_dispersal_of_free-living_marine_nematodes&amp;data=02%7C01%7Cpat.hutchings%40austmus.gov.au%7Cbc9c95b49d284d2d429808d6717a611a%7C6ee75868f5d64c8cb4cda3ddce30cfd6%7C0%7C0%7C636821167775603262&amp;sdata=3lkccxEkQ9lODv17YkmO%2BtKZrXOcBhIw0ZcYIV0Yr0w%3D&amp;reserved=0
>>
>> Regards,
>>
>> Paulo Lana
>>
>> On 03/01/2019 04:21, Pat Hutchings wrote:
>>> Hi folks
>>>
>>> a colleague of mine has asked me if I know any such stains
>>>
>>>
>>> "My colleague has asked me the following question, as I am having very
>> poor luck in a literature search for mark-recapture methods for marine
>> worms. There is an emerging field of work on chemical tracers and stains
>> for the mark-recapture technique, particularly for calcifiers like crabs
>> and molluscs (e.g. using Calcein). Do you know of any people/papers that
>> had success staining worms for recapture and/or successful alternative
>> methods?"
>>>
>>> I had to confess that I had no knowledge of such a stain
>>>
>>>
>>> anyhow if anybody knows - of such stains please can they let me know
>> thanks Pat
>>>
>>>
>>>
>>> Dr Pat Hutchings
>>> Senior Fellow Australian Museum Research Institute
>>>
>>> Visit: https://apac01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.australianmuseum.net.au&amp;data=02%7C01%7Cpat.hutchings%40austmus.gov.au%7Cbc9c95b49d284d2d429808d6717a611a%7C6ee75868f5d64c8cb4cda3ddce30cfd6%7C0%7C0%7C636821167775603262&amp;sdata=0NX0JQqw0GnS8BCdpTKG378kilIIsSQU0cZ9Mfl3q88%3D&amp;reserved=0<
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>>> Inspiring the exploration of nature and cultures
>>> [AM_Logo_Master_RGB_10cm copy.GIF]<
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>>> Australian Museum
>>> 1 William Street
>>> Sydney NSW 2010 , Australia
>>> t 61 2 9320 6243 m 61(0)417486821
>>>
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-- 
Dr. Pei-Yuan Qian
David von Hansemann Professor of Science
Acting Head and Chair Professor, Department of Ocean Science
Chair Professor, Division of Life Science
Hong Kong University of Science and Technology
Clear Water Bay
Hong Kong
Tel: 0852-2358-7331
Fax: 0852-3693 4766
E-mail: boqianpy from ust.hk
Webpage:http://ihome.ust.hk/~pyqian/

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