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[Annelida] anybody able to help - staining worms

Fernanda Oyarzun via annelida%40net.bio.net (by fernanda.oyarzun from gmail.com)
Mon Jan 7 07:27:08 EST 2019


I also used neutral red, in my case for staining spionid larvae and account
for sibling cannibalism. I did trials to see if there was mortality or if
it affected the  development of the larvae before using it, and it had not
effect on those traits. Larvae developed normally.

best wishes,

Fernanda


On Mon, Jan 7, 2019 at 6:56 AM Prof. PY Qian <boqianpy from ust.hk> wrote:

> Just returned to my office from a trip.  I used neutral red for a study
> during my PhD thesis study and the work was published in MEPS in 1994.
> It worked well (without mortality).  PY
>
> .  Pat Hutchings wrote:
> > many thanks will forward it to him Pat
> >
> >
> > Dr Pat Hutchings
> > Senior Fellow Australian Museum Research Institute
> >
> > Visit: http://www.australianmuseum.net.au<
> https://webmail.austmus.gov.au/owa/redir.aspx?C=e7348c0a0d29466da8081316934e9a3b&URL=http%3a%2f%2fwww.australianmuseum.net.au%2f
> >
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> >
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> >
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> >
> > Australian Museum
> > 1 William Street
> > Sydney NSW 2010 , Australia
> > t 61 2 9320 6243 m 61(0)417486821
> > ________________________________
> > From: annelida-bounces from oat.bio.indiana.edu <
> annelida-bounces from oat.bio.indiana.edu> on behalf of Eduardo Zattara <
> ezattara from gmail.com>
> > Sent: Thursday, 3 January 2019 11:44:55 PM
> > To: Annelida from magpie.bio.indiana.edu
> > Subject: Re: [Annelida] anybody able to help - staining worms
> >
> > I have not tried for long term staining worms, but for larger species,
> the
> > fluorescent elastomers used in amphibians might work nicely. The are
> > injected under the skin with a syringe, and tend to stay in place and be
> > visible through the epidermis, so you can combine color dots to achieve
> > group or even individual labeling schemes.
> >
> > Cheers!
> >
> >       -Ed-
> >
> > "Only in silence the word,
> > only in dark the light,
> > only in dying life :
> > bright the hawk's flight on the empty sky"
> >
> > --------------
> > Dr. Eduardo E. Zattara
> >
> > *Adjunct Researcher*
> > Instituto de Investigaciones en Biodiversidad y Medio Ambiente
> > Centro Regional Universitario Bariloche, Universidad Nacional del Comahue
> >
> > *Consejo Nacional de Investigaciones Científicas y Tecnológicas*
> > *Research Collaborator*
> > Dept. of Invertebrate Zoology, National Museum of Natural History
> > *Smithsonian Institution*
> >
> > *Research Associate*
> > Department of Biology, Indiana Molecular Biology Institute
> > *Indiana University, Bloomington*
> >
> > email: ezattara from gmail.com <ezattara from umd.edu>
> > phone: +54 294 461 7958
> > mail address: Quintral 1250, Bariloche, Rio Negro 8400, Argentina
> >
> https://apac01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fsites.google.com%2Fview%2Fecopol%2Fmiembros-del-grupo%2Feduardo-zattara&amp;data=02%7C01%7Cpat.hutchings%40austmus.gov.au%7Cbc9c95b49d284d2d429808d6717a611a%7C6ee75868f5d64c8cb4cda3ddce30cfd6%7C0%7C0%7C636821167775603262&amp;sdata=NOwOHKYQWvLrnd%2FkS7VaynDIvJ%2BcX8pP0ZicNmL80h4%3D&amp;reserved=0
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> >
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> >
> >
> >
> > On Thu, Jan 3, 2019 at 7:04 AM Paulo Lana <lana from ufpr.br> wrote:
> >
> >> Hi Pat and colleagues,
> >>
> >> We have successfully tried Neutral Red  with nematodes. Please have a
> >> look at:
> >>
> >>
> >>
> https://apac01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.researchgate.net%2Fpublication%2F259228576_A_new_look_into_the_small-scale_dispersal_of_free-living_marine_nematodes&amp;data=02%7C01%7Cpat.hutchings%40austmus.gov.au%7Cbc9c95b49d284d2d429808d6717a611a%7C6ee75868f5d64c8cb4cda3ddce30cfd6%7C0%7C0%7C636821167775603262&amp;sdata=3lkccxEkQ9lODv17YkmO%2BtKZrXOcBhIw0ZcYIV0Yr0w%3D&amp;reserved=0
> >>
> >> Regards,
> >>
> >> Paulo Lana
> >>
> >> On 03/01/2019 04:21, Pat Hutchings wrote:
> >>> Hi folks
> >>>
> >>> a colleague of mine has asked me if I know any such stains
> >>>
> >>>
> >>> "My colleague has asked me the following question, as I am having very
> >> poor luck in a literature search for mark-recapture methods for marine
> >> worms. There is an emerging field of work on chemical tracers and stains
> >> for the mark-recapture technique, particularly for calcifiers like crabs
> >> and molluscs (e.g. using Calcein). Do you know of any people/papers that
> >> had success staining worms for recapture and/or successful alternative
> >> methods?"
> >>>
> >>> I had to confess that I had no knowledge of such a stain
> >>>
> >>>
> >>> anyhow if anybody knows - of such stains please can they let me know
> >> thanks Pat
> >>>
> >>>
> >>>
> >>> Dr Pat Hutchings
> >>> Senior Fellow Australian Museum Research Institute
> >>>
> >>> Visit:
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> <
> >>
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> >>
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> <
> >>
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> >>> Inspiring the exploration of nature and cultures
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> >>
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> >>> Australian Museum
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> >>> t 61 2 9320 6243 m 61(0)417486821
> >>>
> >>> [
> >>
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>
> --
> Dr. Pei-Yuan Qian
> David von Hansemann Professor of Science
> Acting Head and Chair Professor, Department of Ocean Science
> Chair Professor, Division of Life Science
> Hong Kong University of Science and Technology
> Clear Water Bay
> Hong Kong
> Tel: 0852-2358-7331
> Fax: 0852-3693 4766
> E-mail: boqianpy from ust.hk
> Webpage:http://ihome.ust.hk/~pyqian/
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-- 
============================================

Fernanda X. Oyarzún, PhD

Investigadora Asociada
Facultad de Ciencias & CIBAS
Universidad Católica de la Ssma. Concepción
http://ciencias.ucsc.cl/persona/fernanda-oyarzun/
fernanda.oyarzun from ucsc.cl

Investigadora Asociada
Centro i~mar, Puerto Montt
Universidad de Los Lagos
http://i-mar2.cl/es/equipo/investigadores-asociados/

Ilustradora Científica y Escultora
Ciencia, Arte & Biodiversidad
www.fernandaoyarzun.com
fernanda.oyarzun from gmail.com


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