<p></p><p class="MsoNormal"><span style="" lang="EN-US">
<br>
<br>
"THE WHITE SEA MOLECULAR ZOOLOGY SUMMER SCHOOL"<br>
at N.A. Pertzov White Sea Biological Station, Moscow State University<br>
September, 6-20 2009<br>
<br>
Announcement<br>
<br>
N.A.Pertzov White Sea Biological Station (WSBS MSU) invites<br>
undergraduate and graduate students studying zoology and ecology to<br>
participate in summer field school on Molecular Zoology, which will<br>
take place in September, 6-20 2009.<br>
<br>
"THE WHITE SEA MOLECULAR ZOOLOGY SCHOOL" will provide a unique<br>
opportunity to get on-hand experience of wide array of molecular<br>
genetics methods and tools used in modern zoology. You are invited to<br>
spend two weeks in marvelous natural settings and friendly environment<br>
at small biological station located in pristine location called<br>
"Velikaya Salma" ("Great straight") of the Kandalaksha Bay
of the<br>
White Sea exactly on the Polar Circle. Addition information about the<br>
station can be found here:<br>
</span><a href="http://wsbs-msu.ru/res/NEWS24/wsbs-presentation.pdf" target="_blank"><span style="" lang="EN-US">http://wsbs-msu.ru/res/NEWS24/wsbs-presentation.pdf</span></a><span style="" lang="EN-US">.<br>
<br>
During two weeks you will be involved in organismal and ecological<br>
fieldwork, laboratory data collection, and analytical work. The course<br>
will emphasise the use of molecular tools to address ecological and<br>
environmental questions.<br>
<br>
The number of participants is limited to 12 people. Working language is English<br>
<br>
Limited financial support to cover accommodation and tuition will be<br>
provided by the Biological Department of M.V. Lomonosov Moscow State<br>
University and Russian Foundation of Basic Research, however travel<br>
and meal expenses will be provided by the participants.<br>
The meal expenses will be near 10 euro per day. The
price of train<br>
ticket from Moscow to WSBS and back will be about 250 euro.<br>
<br>
Syllabus<br>
<br>
<br>
• Faunistic survey of diverse marine fauna in different
biotopes:<br>
littoral fauna, benthic study with grabs and corers, plankton<br>
sampling from research vessel;<br>
<br>
• Washing and processing and preliminary identification of
benthic<br>
samples, introduction to quantitative ecology sampling methods.<br>
<br>
• Overview of major groups of invertebrates inhabiting the
White Sea,<br>
determination of species with taxonomic keys, primer on zoological<br>
drawings.<br>
<br>
• DNA extraction. Overview of applicability of different
protocols.<br>
<br>
• PCR with standard barcoding (COI) primers ,
agarose<br>
electrophoresis, pre-sequencing PCR-product purification<br>
<br>
• PCR-RFLP analysis for species identification<br>
<br>
• PCR with microsatellite (SSR) primers, - acrylamide
electrophoresis,<br>
EtBr staining.<br>
<br>
• Demonstration of other methods such as TA-cloning of PCR
products,<br>
RNA extraction and RT-PCR (optional, subject to availability of time,<br>
reagents, equipment and luck supply)<br>
<br>
• Sequence analysis. Contig construction, quality checking
of<br>
nucleotide sequence, and BLAST and BLAT algorithms.<br>
<br>
• Molecular Genetic databanks (GenBank etc.,) and other
available<br>
resources on the Internet.<br>
<br>
• Discussion Barcoding approaches and approaches of
sequence identification.<br>
<br>
• Introduction to molecular ecology and population
genetics analyses<br>
including estimation of allele frequency of microsatellite loci,<br>
statistical analysis of population differentiation, coalescence<br>
approaches.<br>
<br>
• Introduction to phylogenetic analysis. (PAUP, Mr.Bayes,
PAML, etc.)<br>
<br>
Morning hours lectures (9:30 am – noon will be followed by laboratory<br>
practicals (1 pm till 7 pm), except the days dedicated to full-day or<br>
half-day excursions for specimens collection, either on board of<br>
research vessel "Belomor" or from the shore on littoral zone. Exact<br>
schedule of field trips will be adjusted according to weather and<br>
tides.<br>
<br>
<br>
<br>
Tentative list of lecturers<br>
<br>
Dr. Ken Halianych </span><a href="http://gump.auburn.edu/halanych/lab/" target="_blank"><span style="" lang="EN-US">http://gump.auburn.edu/halanych/lab/</span></a><span style="" lang="EN-US"><br>
Dr. Mikhail Matz </span><a href="http://www.biosci.utexas.edu/ib/faculty/matz.htm" target="_blank"><span style="" lang="EN-US">http://www.biosci.utexas.edu/ib/faculty/matz.htm</span></a><span style="" lang="EN-US"><br>
Dr. Alex Kondrashov </span><a href="http://www.eeb.lsa.umich.edu/eeb/people/kondrash/index.html" target="_blank"><span style="" lang="EN-US">http://www.eeb.lsa.umich.edu/eeb/people/kondrash/index.html</span></a><span style="" lang="EN-US"><br>
Dr.Tatiana Azhikina (Institute of Bioorganic Chemistry, Moscow)<br>
</span><a href="http://humgen.siobc.ras.ru/people/people.html" target="_blank"><span style="" lang="EN-US">http://humgen.siobc.ras.ru/people/people.html</span></a><span style="" lang="EN-US"><br>
Dr.Tatiana Neretina (WSBS) </span><a href="http://wsbs-msu.ru/doc/index.php?ID=22" target="_blank"><span style="" lang="EN-US">http://wsbs-msu.ru/doc/index.php?ID=22</span></a><span style="" lang="EN-US"><br>
Dr.Anna Zhadan (WSBS) </span><a href="http://wsbs-msu.ru/doc/view.php?ID=44" target="_blank"><span style="" lang="EN-US">http://wsbs-msu.ru/doc/view.php?ID=44</span></a><span style="" lang="EN-US"><br>
Prof. Yuriy Lebedev (Institute of Bioorganic Chemistry, Moscow)<br>
</span><a href="http://labcfg.ibch.ru/index.html" target="_blank"><span style="" lang="EN-US">http://labcfg.ibch.ru/index.html</span></a><span style="" lang="EN-US"><br>
Dr. Nikolai Mugue (Institute of Developmental Biology RAS / VNIRO,<br>
Moscow) </span><a href="http://idbras.comcor.ru/indexe.HTM" target="_blank"><span style="" lang="EN-US">http://idbras.comcor.ru/indexe.HTM</span></a><span style="" lang="EN-US"><br>
<br>
Contact e-mail: </span><a href="mailto:wsbs.school@gmail.com"><span style="" lang="EN-US">wsbs.school@gmail.com</span></a><span style="" lang="EN-US"><br>
Contact person: Tatyana Neretina<br>
For participation in this school please fill the form and send it<br>
before the 30th of March, 2009.<br>
<br>
<br>
PRE-REGISTRATION FORM<br>
<br>
Name ………………………………………………………………………..<br>
<br>
Affiliation...........................................................................................................<br>
<br>
Taken classes ………………………………………………………………….<br>
<br>
Experience of fieldwork …………………………………………………………<br>
<br>
</span>Why are you interesting in this summer school?</p>
