Dear collegues<br> I wonder if anyone ever tried to isolate mitochondrias from earthworms for posterior mtDNA extraction, could anyone sen me a good protocol? I am having a lot of trouble doing that, probably due to soil contamination in the digestive tract, even after depuration and I am getting very low yield of mtDNA. Any help?? I am working with Pontoscolex corethrurus, my model organism :).<br>
<br>Best regards<br clear="all"><br>-- <br>Luís Cunha <br>(Msc, Phd student)<br>Centro de Investigação em Recursos Naturais, Departamento de Biologia – Universidade dos Açores, Rua da Mãe de Deus, 13A – Apartado 1422, 9501-855 Ponta Delgada<br>
