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<div style="direction:ltr; font-family:Tahoma; color:#000000; font-size:10pt">My view is that, like a visit to the dentist, formalin fixation is an unpleasant but necessary process for long term protection from decay.&nbsp; Ethanol as a fixative at proper concentration
 renders specimens so rigid and brittle they are almost unworkable with - except for DNA extraction, and at lower concentrations you can get unidentifiable mush.&nbsp; Proprietory fixative formulas of unknown recipe are around. Trust a secret recipe - maybe not
 - and price might be a limitation.<br>
<br>
Geoff<br>
<br>
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<div id="divRpF755194" style="direction:ltr"><font color="#000000" face="Tahoma" size="2"><b>From:</b> annelida-bounces@oat.bio.indiana.edu [annelida-bounces@oat.bio.indiana.edu] on behalf of Doug Strom [dstrom@waterandair.com]<br>
<b>Sent:</b> 25 February 2012 10:15<br>
<b>To:</b> Polychaete mailing list<br>
<b>Subject:</b> Re: [Annelida] substitution for formalin<br>
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<div>The USEPA has started using a product called NOTOXHisto:<br>
<br>
<a href="http://www.scientificdevice.com/notoxhisto/" target="_blank">http://www.scientificdevice.com/notoxhisto/</a><br>
<br>
For the samples we have seen it appears to fix macroinvertebrates and in particular polychaetes pretty well.<br>
<br>
Doug<br>
<br>
[I sent this reply to Barbara, but meant it for the group as well. - DGS]<br>
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<span>Doug Strom</span><br>
<span>Project Scientist - Aquatic Ecologist<br>
</span><span><span style="font-family:arial,helvetica,sans-serif">Water &amp; Air Research Inc.</span><br style="font-family:arial,helvetica,sans-serif">
<span style="font-family:arial,helvetica,sans-serif">6821 SW Archer Road</span><br style="font-family:arial,helvetica,sans-serif">
<span style="font-family:arial,helvetica,sans-serif">Gainesville, Florida 32608</span><br style="font-family:arial,helvetica,sans-serif">
<span style="font-family:arial,helvetica,sans-serif">Toll Free: <a href="tel:800.242.4927" target="_blank">
800.242.4927</a> </span><br style="font-family:arial,helvetica,sans-serif">
<span style="font-family:arial,helvetica,sans-serif">Local: <a href="tel:352.372.1500" target="_blank">
352.372.1500</a> </span><br style="font-family:arial,helvetica,sans-serif">
<span style="font-family:arial,helvetica,sans-serif">Fax: <a href="tel:352.378.1500" target="_blank">
352.378.1500</a><br>
</span></span><span><a href="http://www.waterandair.com/" target="_blank" style="color:blue; text-decoration:none">dstrom@waterandair.com</a></span><br>
<span><a href="http://www.waterandair.com/" target="_blank" style="color:blue; text-decoration:none">www.waterandair.com</a>
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<div class="gmail_quote">On Fri, Feb 24, 2012 at 5:50 AM, Barbara Mikac <span dir="ltr">
&lt;<a href="mailto:barbara.mikac@cim.irb.hr" target="_blank">barbara.mikac@cim.irb.hr</a>&gt;</span> wrote:<br>
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Dear colleagues,<br>
<br>
I am interested if there is some other fixative for the macrozoobenthic<br>
samples that can substitute formalin? All groups will be analysed<br>
analyses later but special interest will be in polychaetes.<br>
<br>
Thank you very much for your help!<br>
<br>
Barbara<br>
<br>
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