Postdoctoral Position available
JONESJ at jii.afrc.ac.uk
JONESJ at jii.afrc.ac.uk
Tue Jul 23 05:41:00 EST 1991
Postdoctoral Position available.
An exciting post doctoral research position is available in
the Sainsbury Laboratory, at the John Innes Centre
in Norwich, to study the interaction between our favorite
weed, A. thaliana, and one of its most merciless oppressors,
the oomycete fungus, Peronosopora parasitica. (see Koch and
Slusarenko(1990), Plant Cell 2 437-445).
This project got started as follows.
In the course of studies by Jane Parker and Mike Daniels
on segregation in Arabidopsis for a response to
Xanthomonas, a Peronospora infection arose that was of a race
with a previously undetected specificity, namely it was virulent
on Columbia but not on Landsberg. Studies in collaboration with
Ian Crute and Eric Holub in East Malling suggest the presence
of segregating genes for resistance in the F2.
At the John Innes Centre in Norwich, the lab of Caroline Dean
has developed F8 recombinant inbreds (see Burr, TIG, Feb 91) of
Landsberg/Columbia which will be screened using commercially
available 10mer primers (from Operon Technologies, Alameda CA).
A high resolution RAPD locus (NAR 18 6531-6535) linkage map
will be developed using this recombinant inbred material,
and tight linkage will be sought to the resistance gene(s). The next
objective will of course be to attempt to walk to the R gene (s), in
order to clone them and try and figure out how they work. We expect
that walking should be quite efficient. Initial detection of linkage
should be fast with RAPDs. Two labs here (C Dean,
G Coupland) are already walking at a pretty good rate and all the
technology is in place to both walk and.subclone from YACs
into binary cosmid vectors for complementation experiments.
The successful candidate will
(i) score the recombinant inbreds for resistance/sensitivity
to the Norwich race of Peronospora parasitica (this in collaboration
with Ian Crute/Eric Holub)
(ii) participate in making a RAPD map using the recombinant
inbreds, and in correlating it with the RFLP map.
(iii) identify loci/probes/YACs that are closely linked to the
(iv) attempt complementation experiments using closely linked
DNA cloned into binary vectors.
Although the primary objective is to accomplish the molecular
genetics objectives described above, there is scope for some biological
studies looking in more detail at the interaction, and for seeking new
isolates of the fungus and new resistance genes in local natural
Arabidopsis and Peronospora populations.
If interested, please contact Jonathan Jones, The Sainsbury
Laboratory, John Innes Centre, Colney, Norwich NR4 7UH. Applicants
should send their CV and the names and addresses of 3 referees as soon
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