We have isolated a mutant which is sensitive to cadmium. The phenotype
is expressed in callus tissue and would aqppear to be a reasonable
candidate for cloning by complementation using a library in a binary
vector constructed either from YACs in the region of the gene or DNA of
the appropriate size identified using nearby RFLPs and purified from a
CHEF gel. Has anyone tried this approach for another selectable gene? Can
anyone suggest a useful vector, preferably not subject to proprietary
rights, for this approach? I would be grateful for any advice, please.