Biolistic Transformations
Peter Quail
pquail at ENZYME.BERKELEY.EDU
Mon May 6 14:43:28 EST 1991
In response to Chris Town's question about bombarding Arabidopsis, I have
used the Dupont Biolistic gun for this purpose. I used a standard DNA
and particle preparation protocol and shot 2, 3, and 4 day old etiolated
seedlings as well as 3-week old green plants. The 2 day seedlings worked
somewhat better than the 3 or 4 day old ones. I did not try earlier
stages. Feel free to contact me directly (415-559-5916) for further
details.
Tim Caspar
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