unidentified band

BI1RGW at IBM.SHEFFIELD.AC.UK BI1RGW at IBM.SHEFFIELD.AC.UK
Fri Nov 15 07:05:35 EST 1991


My question produced a stream of answers/suggestions. Many thanks to those who
answered. To briefly summarise:

Several people suggested that 1% Triton was extracting intrinsic membrane
proteins: this is not the case - although the membranes are broken up, they are
still in *suspension* rather than solution, and are pelleted by centrifugation.
The Triton does extract all the chlorophyll, though.

Other suggestions included: peroxidase, acid phosphatase or aldolase. However,
none of these would be present in the sort of quantities I am seeing.

The answer is, of course, blindingly obvious - "ACTIN !!!" (this was the full
text of the answer from prizewinner David Lonsdale (LONSDALE at AFRC.JII). No
other soluble protein could conceivably compete with RUBISCO is terms of
quantity.

Robin Walters.

PS - the 50kDa band was one subunit of the chloroplast ATPase CFo, which the
Triton presumably *does* strip off the membrane.



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