Unidentified Band

[BI1RGW at IBM.SHEFFIELD.AC.UK]

Can anyone out there answer this question?
What fairly major protein of approximate MW 40kDa is found in the soluble
protein fraction after grinding Arabidopsis leaves in a buffer containing
1% v/v Triton X-100? Apart from RUBISCO large and small subunits, this is the
strongest band on an SDS-PAGE gel stained with Coomassie Blue.
Any guesses?

There's also another fairly strong band of around 50kDa which we haven't
identified - suggestions for candidates for that would also be welcome.

Thanks.

Robin Walters            Robert Hill Institute,
BI1RGW at UK.AC.SHEF.IBM    Dept. of Mol. Biol. & Biotech.,
(if you're outside       University of Sheffield,
the UK reverse the       P.O.Box 594,
elements of the node-    Western Bank,
name)                    Sheffield S10 2UH.
                         United Kingdom