(none)

LEHLEF at CCIT.ARIZONA.EDU LEHLEF at CCIT.ARIZONA.EDU
Thu Oct 24 00:11:00 EST 1991


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October 23, 1991

TO:       ARABIDOPSIS RESEARCHERS
FROM:     FRED LEHLE
RE:       FAST NEUTRON IRRADIATED ARABIDOPSIS SEED

As interest appears high, I thought it appropriate to mention that LEHLE
SEEDS expects to have fast neutron irradiated M2 seed available
sometime in January 1992.  M2 seed will be available in three
backgrounds, Columbia, Landsberg erecta, and RLD and will be bulked
in parental groups of about 1000 M1 parents.

The irradiation dosage of the seed to be offered was 60 Gy and was
performed by the IAEA in Vienna Austria.  Seeds were irradiated dry in
paper envelopes.  I have grown out a small sample of these seeds
(Landsberg erecta background only) and have made the following
preliminary observations.

Establishment is about 15% of untreated controls.  I define
establishment as the percent of seeds which produce a fertile M1 parent.
I estimated this by germinating the seeds on nutrient agar plates and
then transferring all viable seedlings to potting mix.

I also selected 4 siliques each from fertile M1 parents and counted the
number which showed segregation for albino embryos.   It is my
understanding that albino embryos are rarely seen in unmutagenized
material.  The work is still in progress, but 16 out of 78 (20%) of the
fertile M1 parents exhibit segregation of albino embryos among green
embryos.

If you wish to be notified as soon as these fast neutron irradiated seeds
are offered, kindly send a card, letter, or FAX with your name and
address to the following location.  

LEHLE SEEDS
6531 North Camino Katrina
Tucson AZ 85718 USA
Phone 602-544-0733
FAX 602-797-9009 (THIS IS OUR NEW FAX NUMBER)

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