subcellular fractionation

Joel A. Kreps jak8 at po.CWRU.Edu
Fri Apr 17 14:17:56 EST 1992


Dear Network,		April 17,

I tried sending this message through the Arab. network but somet
hing seems to have been fouled up.  I would like to find out what
methods people are using to fractionate cellular proteins into
nuclear and cytosolic fractions for use in generating western 
blots for probing with antibodies.  I have several animal methods 
but I want to know the methods which are currently being used
with plants.  Thanks.

Joel A. Kreps (in Chris Town's lab)



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