Isolation of RNa from seeds

TAGUE at FRODO.MGH.HARVARD.EDU TAGUE at FRODO.MGH.HARVARD.EDU
Tue Aug 25 14:47:29 EST 1992


Does anyone have a reliable method for the isolation of RNA from dry seeds 
and/or yellow siliques. With the standard RNA prep (SDS/LiCl/phenol/chloroform)
some kind of contaminant comes through with the RNA. Procedures for the removal
of polysaccharides (1M NaCl ppt @ 0C, acid ppt) don't remove it. Any hints or
suggestions would be greatly appreciated. Thanks in advance.
-- Brian W. Tague



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