lea or other seed promoters.

ELIZ at CCIT.ARIZONA.EDU ELIZ at CCIT.ARIZONA.EDU
Tue Dec 8 18:29:12 EST 1992


Networkers,
	We have found that small HSPs are expressed in developing seeds in a
pattern similar to LEA proteins, that is they are made late in development
and stored in the dry seed and decline during imbibition.  We would like
to use antisense RNA to specifically knock out their expression under these conditions (or at this phase of the life cycle).  Can anyone recommend a suitable
promoter to use for this?  Might 35S work, or would we be better off using
a lea promoter, and does anyone have such a thing for Arabidopsis?  Any
input would be appreciated.
Elizabeth Vierling
University of Arizona
ELIZ at ccit.arizona.edu



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