We have tried unsuccessfully to transform an Arabidopsis tumor cell
line for the last couple of months using Agrobacterium methods. One
question that has arisen is whether our DNA is getting into the cells
at all. Since we were informed that Arabidopsis protoplasts are easily
transformed by the PEG method, we have decided to use this alternate
transformation procedure. Before we actually attempt the transformation,
we thought we would spend the time to determine whether we can get any
DNA expressed in our cell line. My questions to the network are:
1.) Are there any published papers using PEG transformation and transient
GUS expression to examine Arabidopsis?
2.) Is there anyone out there who has transformed Arabidopsis with GUS
using the PEG method who has not published? How many protoplasts
did you use in the transformation? How much plasmid DNA did you use?
3.) Does anyone have seeds for a transgenic GUS plant that they would
be willing to send us? These seeds would help us to develop our GUS
thanx to all.
Jim Campanella|"Iron Man I, how do you copy?"
CWRU Bio Dept.|"With a Xerox machine. And you?"
Cleveland, | -Tom Servo, Marooned, MST3K