hygromycin vector

thomas altmann altmann at mpimg-berlin.mpg.dbp.de
Sun Mar 29 20:01:00 EST 1992


We are using hygromycin selection mainly for Arabidopsis transformation. Three different vectors are in use in our lab: The pGV3850HPT (Baker et al., EMBO J. 6, 1547-1554, 1987) is a cointegrate-type vector that requires cloning into a pBR-derived intermed
iate plasmid. The pGSC1704 (to be requested from PGS, Gent)and the pBIB-HYG (Becker, NAR 18, 203, 1990) are binary vectors that work well in our leaf disc transformation procedure.
Thomas Altmann, IGF Berlin



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