Isolation of vacuoles from Arabidopsis

[William D. Graziadei]

In a message from Chris Cobbett, he asked....

>From:	IN%"U6525069 at ucsvc.ucs.unimelb.edu.au" 12-MAR-1992 18:46:49.24
>
>Does anyone have a method for purifying vacuoles adapted to Arabidopsis?
>Methods for any other plants might be useful - any information gratefully
>received.
--------------------------------------------------------------------------------
Chris, you might look at:

REFERENCE:     Kreis,W., and Reinhard,E.  1986.  Selective Uptake
and Vacuolar Storage of Primary Cardiac Glycosides by Suspension-
cultured Digitalis lanata Cells.  J PLANT PHYSIOL 128 pp.311-326.

One of our students is doing his plant research project on cardiac
glycosides and cites the above as his key reference.


   Regards,

      Bill 

   Question: What is remedial mitosis?

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"Remedial mitosis is a math course for cells that can't multiply or divide."
Instructor: Dr. G. Factor; Sections: G1, G0 (Remedial), S, G2, M; Time: Varies