GUS assay

[George Karlin-Neumann]

Neil,

    Chlorophyll can be removed (after GUS staining) by incubation of tissue in
successively higher concentrations of EtOH (e.g. 30%, 50% 70%) for perhaps 1 or
2 hr per incubation, or overnight if more convenient.  To fully bleach the
tissue, several incubations in 70% EtOH may be necessary, depending on volume
and tissue mass used.  The GUS staining appears to be stable to this treatment
and stained tissue can be preserved in 70% EtOH for many months.

                                        George Karlin-Neumann

                                        Biochem Dept, Stanford 
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