T-DNA pools

Randall L Scholl rscholl at magnus.acs.ohio-state.edu
Thu May 28 14:10:58 EST 1992



SEED TRANSFORMANT POOLS READY FOR DISTRIBUTION - ABRC AT OHIO STATE
-------------------------------------------------------------------

A. GENERAL DESCRIPTION OF STOCKS

     Transformants,4900 in number, were independently isolated
after Arabidopsis seed was treated with Agrobacterium. Lines were
established from these through self-fertilization by Dr. Ken
Feldmann at the University of Arizona. Seeds from groups of these
lines were pooled into lots consisting of either 100 or 20 lines
per pool. Hence, the population is available for mutant screening
as either 49 size-100 pools and 245 size-20 pools. 

B. DISTRIBUTION POLICIES

     The distribution procedure for these stocks will be as
follows: While two types of pools are available, investigators are
encouraged to order the size-100 pools for all screens for which
they can be used.  These should be most convenient for all but the
most difficult screens.   The associated size-20 pools for each
larger pool will be available on an individual basis for
rescreening, if they are needed by the investigator.  For those
individuals who absolutely require the smaller pool size, the size
20 pools will be provided.  Initially, no charge for receiving any
of the material will be assessed.  However, if excessive demand
occurs, charges may have to be made, especially in the case of the
size-20 pools.

C. ORDERING OF POOLS AND STOCK LIST

     The size-100 pools can be ordered as follows: E-mail, mail or
fax an order (see below or in our April, 1992 stock list)
requesting stocks CS-2606 through CS-2654.  The reference
information relating these stocks to the original Feldmann material
is detailed in the following table.  The five size-20 pools
associated with each size-100 pool are listed in the table as are
the original Feldmann lines from which each was derived.  The
visible mutants that have already been isolated from each pool can
be ascertained by comparing the line numbers of the table with the
line numbers of individual mutants in our April, 1992 stock list. 
We currently possess approximately 150 individual mutants, and
expect to receive several hundred more from the Feldmann lab within
several weeks.  

     These stocks will be available to European investigators from
the Nottingham Arabidopsis Stock Centre.  


  Feldmann Seed Transformants - Pools of 100 lines each               

---------------------------------------------------------------
Stock #   Size-20        Corresponding
of pool   CS Stock #     s    Feldmann T3 #s 
---------------------------------------------------------------
CS-2606   2361 - 2365    1-100
CS-2607   2366 - 2370    101-200
CS-2608   2371 - 2375    201-220, Z1-Z20, 241-300
CS-2609   2376 - 2380    301-320, Z21-Z40, 341-400
CS-2610   2381 - 2385    401-440, Z41-Z60, 461-500
CS-2611   2386 - 2390    501-600
CS-2612   2391 - 2395    601-700
CS-2613   2396 - 2400    701-800
CS-2614   2401 - 2405    Z61-Z100, 841-900
CS-2615   2406 - 2410    901-1000
CS-2616   2411 - 2415    1001-1100
CS-2617   2416 - 2420    1101-1160, Z101-Z120, 1181-1200
CS-2618   2421 - 2425    1201-1300
CS-2619   2426 - 2430    1301-1360, Z121-Z160
CS-2620   2431 - 2435    1401-1500
CS-2621   2436 - 2440    1501-1520, 1541-1560,Z161-Z200,1581-1600
CS-2622   2441 - 2445    Z201-Z220, 1621-1700
CS-2623   2446 - 2450    1701-1800
CS-2624   2451 - 2455    1801-1900
CS-2625   2456 - 2460    1901-1940, Z221-Z240, 1961-2000
CS-2626   2461 - 2465    2001-2100
CS-2627   2466 - 2470    2101-2200
CS-2628   2471 - 2475    2201-2240, Z241-Z260, 2261-2300
CS-2629   2476 - 2480    2301-2400
CS-2630   2481 - 2485    2401-2500
CS-2631   2486 - 2490    2501-2580, Z261-Z280
CS-2632   2491 - 2495    2601-2680, Z241-Z260
CS-2633   2496 - 2500    2701-2780, Z281-Z300
CS-2634   2501 - 2505    2801-2900
CS-2635   2506 - 2510    2901-3000
CS-2636   2511 - 2515    3001-3020, Z301-Z320, 3041-3100
CS-2637   2516 - 2520    3101-3200
CS-2638   2521 - 2525    3201-3300
CS-2639   2526 - 2530    3301-3400
CS-2640   2531 - 2535    3401-3500
CS-2641   2536 - 2540    3501-3600
CS-2642   2541 - 2545    3601-3700
CS-2643   2546 - 2550    3701-3800
CS-2644   2551 - 2555    Z321-Z420
CS-2645   2556 - 2560    3901-4000
CS-2646   2561 - 2565    4001-4100
CS-2647   2566 - 2570    Z421-Z440, 4121-4200
CS-2648   2571 - 2575    4201-4240, Z441-Z460, 4261-4300
CS-2649   2576 - 2580    4301-4400
CS-2650   2581 - 2585    4401-4500
CS-2651   2586 - 2590    4501-4600
CS-2652   2591 - 2595    4601-4700
CS-2653   2596 - 2600    4701-4800
CS-2654   2601 - 2605    4801-4900
-----------------------------------------------------------------               

     The full set of size-20 pools can obtained by ordering Stocks
CS-2361 through CS-2605.  These also correspond to Feldmann lines
as indicated in the above table.


E. SCHEDULE OF SHIPMENT FOR THESE STOCKS

     The size-100 pools are available for immediate shipment. Some
orders have already been received, and these will be shipped
immediately. It should be possible to fill new orders for these
stocks as they arrive.  Aliquoting of an initial group of size-20
pools is still in progress. Completion of this process and
shipment of the first size-20 stocks should occur by June 6.

F. ORDERING CONTACTS

     mail:          Arabidopsis Biological Resource Center at Ohio
                    State
               1735 Neil Avenue
               Columbus, OH 43210, USA

     fax:      614-292-0603

     e-mail:   seeds at genesys.cps.msu.edu (for seed orders).
               dna at genesys.cps.msu.edu (for DNA orders).

NOTE:  For  ALL e-mail orders, type "STOCK ORDER" in the Subject
line! This will allow our computer system to automatically direct
it toward stock ordering.

The Center's telephone numbers are:

main number:   614-292-9371        (seed ordering information)
Randy Scholl:  614-292-1982        (for general and seed-related
                                        questions)
Keith Davis:   614-292-2115        (for DNA-related questions)
e-mail:arabidopsis+ at osu.edu



More information about the Arab-gen mailing list