in planta transformation
mb1rgw at sunc.sheffield.ac.uk
Tue Nov 17 12:21:07 EST 1992
We are interested in trying out the _in planta_ transformation which has been
posted by Chris Somerville's lab. Unfortunately, having less than zero
knowledge of plant anatomy, we would appreciate some assistance:
"When the plants start to bolt (flowering shoot <2cm), we cut the flowering
shoot and all visible axillary buds with a forceps, or a razor blade."
(1) By "cut", does it mean cut _off_, or just make a nick in the side?
(2) What is an axillary bud?
Thanks for any help (and additional tips on using the method?).
Robert Hill Institute, University of Sheffield, UK.
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