pollen mutagenesis

LevinJ levinj at STARBASE1.CALTECH.EDU
Tue Sep 15 22:51:48 EST 1992


I would like to find out about mutagenesis of Arabidopsis pollen.
What dosages and types of radiation have been most successful in producing
mutations that can be recovered in subsequent generations?  Is there a
documented difference in the spectrum of DNA changes produced by x rays, gamma
rays, and other radiation in Arabidopsis or other plants?  Is there a mutagenic
treatment of pollen that will produce predominantly point mutations and small
deletions?  Any references or reports from first-hand experience will be
appreciated.



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