southern hybe/wash conditions

clincoln at INSECT.BERKELEY.EDU clincoln at INSECT.BERKELEY.EDU
Mon Apr 12 14:42:04 EST 1993


We would like suggestions for low stringency hybridization and wash
conditions for genomic southern blots hybridized with heterologous
probes...for example, an Arabidopsis southern blot hybridized with a maize
gene probe. Conditions which give the lowest background hybridization are
obviously most desirable. Protocols (or references to procedures) with
comments describing why a particular set of conditions were used would be
helpful.
thanks.
Cindy Lincoln and Lauren Hubbard




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