PCR differences between ecotypes

Nadine Smith nmsmith at sunflower.bio.indiana.edu
Sat Apr 17 15:05:28 EST 1993

	I am using PCR to map a gene and I am having trouble getting
	the PCR to work for several different ecotypes. It worked
	beautifully with Columbia and Bla-2, but I can't get the
	same primers (same exact protocol) to amplify La-0 and
	a Col-0 mutant (generated with fast neutrons). Does anybody
	have any experience with this or any possible remedies?
	Any suggestions at all will be greatly appreciated.
				Nadine Smith, Indiana University Biology

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