PCR differences between ecotypes

Nadine Smith nmsmith at sunflower.bio.indiana.edu
Sat Apr 17 15:05:28 EST 1993


	I am using PCR to map a gene and I am having trouble getting
	the PCR to work for several different ecotypes. It worked
	beautifully with Columbia and Bla-2, but I can't get the
	same primers (same exact protocol) to amplify La-0 and
	a Col-0 mutant (generated with fast neutrons). Does anybody
	have any experience with this or any possible remedies?
	Any suggestions at all will be greatly appreciated.
				
				Thanks,
				Nadine Smith, Indiana University Biology



More information about the Arab-gen mailing list